Expression of a 74-kDa nuclear factor 1 (NF1) protein is induced in mouse mammary gland involution. Involution-enhanced occupation of a twin NF1 binding element in the testosterone-repressed prostate message-2/clusterin promoter.

The Journal of Biological Chemistry
E E FurlongF Martin

Abstract

Testosterone repressed prostate message-2 (TRPM-2)/clusterin gene expression is rapidly induced in early involution of the mouse mammary gland, after weaning, and in the rat ventral prostate, after castration. A search for involution-enhanced DNaseI footprints in the proximal mouse TRPM-2/clusterin gene promoter led to the identification and characterization (by DNase I footprinting and EMSA) of a twin nuclear factor 1 (NF1) binding element at -356/-309, relative to the proposed transcription start site; nuclear extracts from 2-day involuting mouse mammary gland showed an enhanced footprint over the proximal NF1 element; extracts from involuting prostate showed enhanced occupancy of both NF1 binding elements. Subsequent EMSA and Western analysis led to the detection of a 74-kDa NF1 protein whose expression is triggered in early involution in the mouse mammary gland; such an induced protein is not found in the involuting rat ventral prostate. This protein was not found in lactation where three other NF1 proteins of 114, 68, and 46 kDa were detected. Reiteration of the epithelial cell apoptosis associated with early mammary gland involution, in vitro, in a primary cell culture system, triggered the appearance of the 74-kDa NF1. O...Continue Reading

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Citations

Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·L das NevesF Godinho
Aug 4, 2004·Cellular and Molecular Life Sciences : CMLS·K A Green, C H Streuli
May 22, 2007·Biochemical and Biophysical Research Communications·Sudit S Mukhopadhyay, Jeffrey M Rosen
Apr 25, 2000·Cell Death and Differentiation·F C O'Connell, F Martin
Apr 1, 2014·Molecular Endocrinology·Gertraud W RobinsonLothar Hennighausen
Mar 6, 2002·The Journal of Biological Chemistry·Marie Kannius-JansonJeanette Nilsson
May 7, 2002·The Journal of Biological Chemistry·Rosemary KaneFinian Martin
Apr 25, 2000·The Journal of Biological Chemistry·R W ClarksonC J Watson
Jul 20, 1999·Molecular and Cellular Endocrinology·R Bailey, M D Griswold
Nov 18, 2010·Journal of Medicinal Chemistry·Niamh M O'BoyleMary J Meegan

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