Expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants.

Molecular Plant Pathology
Yoshimitsu TakakuraHidemasa Imaseki

Abstract

Flagellin is a component of bacterial flagella and acts as a proteinaceous elicitor of defence responses in organisms. Flagellin from a phytopathogenic bacterium, Acidovorax avenae strain N1141, induces immune responses in suspension-cultured rice cells. To analyse the function of flagellin in rice, we fused the N1141 flagellin gene to the cauliflower mosaic virus 35S promoter and introduced it into rice. Many of the resulting transgenic rice plants accumulated flagellin at various levels. The transgenic rice developed pale spots in the leaves. The expression of a defence-related gene for phenylalanine ammonia-lyase was induced in the transgenic plants, and H(2)O(2) production and cell death were observed in some plants with high levels of gene expression, suggesting that the flagellin triggers immune responses in the transgenic rice. Transgenic plants inoculated with Magnaporthe grisea, the causal agent of rice blast, showed enhanced resistance to blast, suggesting that the flagellin production confers disease resistance in the transgenic rice.

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Citations

Aug 21, 2010·Molecular Biotechnology·Louis-Philippe Bergeron-SandovalFathey Sarhan
Jan 13, 2012·Molecular Plant-microbe Interactions : MPMI·Anna BuschartBarbara Reinhold-Hurek
Feb 4, 2009·Trends in Biotechnology·Pari Skamnioti, Sarah J Gurr
Jun 2, 2010·Plant Signaling & Behavior·Marcelo O Santos, Francisco J L Aragão
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Ravinder K Goyal, Autar K Mattoo
Jun 9, 2016·PloS One·Laura HelftAndrew F Bent
Dec 23, 2016·Scientific Reports·Terry MunStig U Andersen

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