PMID: 8946920Nov 1, 1996Paper

Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos

Genes & Development
S HopplerR T Moon

Abstract

During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to differentiate in vitro as ventral mesoderm, no loss-of-function studies have demonstrated a requirement for Wnt signaling for the normal expression of mesodermal genes in the gastrula. We report development of a dominant-negative Wnt (dnXwnt-8) that inhibits embryonic responses to Wnt signaling in a cell-nonautonomous fashion. By expressing dnXwnt-8 in Xenopus embryos, we uncover a requirement of Wnt signaling for localized expression in prospective mesoderm of XMyoDa and Xenopus-posterior (Xpo). Because ectopic expression of functional Xwnt-8 in the dorsal marginal zone of the gastrula induces ectopic XMyoDa and Xpo, both gain-of-function and loss-of-function experiments support a model in which endogenous Xwnt-8 functions to induce expression of genes involved in specification of ventral and somitic mesoderm.

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Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R T Moon, D Kimelman
Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Bouwmeester, L Leyns
Sep 26, 2002·Developmental Biology·Alin Vonica, Barry M Gumbiner
Oct 25, 2008·Development Genes and Evolution·Laura Beaster-JonesLinda Z Holland
Feb 20, 2003·Developmental Biology·Arne C LekvenRandall T Moon
Jul 30, 2003·Developmental Biology·Stella M HonoréRoberto Mayor
Jul 25, 2003·Journal of Molecular and Cellular Cardiology·Tarek KashourFrancis Amara
Feb 19, 2002·Mechanisms of Ageing and Development·Jane M Taylor-JonesCharlotte A Peterson
Jun 27, 2002·Biochimica Et Biophysica Acta·Yosef LandesmanDavid L Paul
Apr 1, 1997·Trends in Genetics : TIG·R T MoonM Torres
Aug 4, 1999·Trends in Genetics : TIG·C Niehrs
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