Expression of a down-regulated target, SSeCKS, reverses v-Jun-induced transformation of 10T1/2 murine fibroblasts

Oncogene
Steven B CohenP K Vogt

Abstract

Line 10T1/2 mouse fibroblast overexpressing the v-Jun oncoprotein were morphologically altered, grew into multilayered foci in culture and formed colonies when suspended in agar. The growth rate of the v-Jun-transformed 10T1/2 cells was not changed significantly from that of the untransformed parental cells, but the saturation density of the transformed cultures exceeded that of normal controls by a factor of 2. mRNA extracted from v-Jun-transformed 10T1/2 cells was analysed for differential gene expression with DNA micro-array technology. One of the targets downregulated by v-Jun was identified as SSeCKS (Src-suppressed C kinase substrate). Re-expression of SSeCKS in v-Jun-transformed fibroblasts reversed the transformed phenotype of the cells. Their ability to form foci was reduced to background levels, the number and size of agar colonies was lowered by a factor of 10 and the saturation density was significantly diminished. However, expression of SSeCKS had little effect on the morphology of v-Jun-transformed 10T1/2 cells. These data suggest that the SSeCKS protein has growth-attenuating properties. Down-regulation of SSeCKS may be essential for Jun-induced transformation.

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Citations

Jun 4, 2008·Oncogene·H Jiang, P K Vogt
Jul 3, 2008·Cancer Research·Shin AkakuraIrwin H Gelman
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yutaka MiuraSenitiroh Hakomori
Sep 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sumit K ChandaJeremy S Caldwell
Jun 17, 2008·Biochemical and Biophysical Research Communications·Moon-Chang ChoiYung-Jue Bang
Oct 11, 2011·Bioscience Reports·Gautam SethiVinay Tergaonkar
Jun 27, 2006·Oncogene·B W OzanneR F Hennigan
Jun 13, 2001·Oncogene·P K Vogt
Sep 18, 2007·The Journal of Primary Prevention·Joseph R FerrariVictoria Lyman
Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young Yun JungKwang Seok Ahn

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