PMID: 19912815Aug 1, 1991Paper

Expression of a functional foreign gene in adult mammalian brain following in Vivo transfer via a herpes simplex virus type 1 defective viral vector

Molecular and Cellular Neurosciences
M G KaplittD W Pfaff

Abstract

We report here the first use of a herpes simplex virus defective viral vector for the transfer and expression of a foreign gene in the adult rat brain in vivo. Defective vectors offer unique advantages over other systems. Our vector genome consists of multiple copies of a plasmid-based amplicon, with a human cytomegalovirus promoter and lacZ gene as a reporter. Helper functions were provided by an HSV1 mutant incapable of replication at physiological temperatures. The resulting defective viral vector was stereotaxically microinjected into rat hippocampus and hypothalamus, and cells expressing functional beta-galactosidase were detected in both areas. Expression was confined to regions at or near the site of injection. Positive cells were identified by 18 hr following injection, and expression was still detectable after 2 weeks. All animals survived with no behavioral, gross, or microscopic anatomical evidence of a virulent neurologic infection.

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Citations

Nov 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L C Doering
Jan 1, 1994·Journal of Neuro-oncology·M G KaplittR G Blasberg
Dec 1, 1993·Journal of Neuroscience Methods·P Casaccia-BonnefilP J Bergold
Apr 1, 1995·Journal of Neuroscience Methods·D Y HoR M Sapolsky
Apr 1, 1996·Brain Research. Molecular Brain Research·K C New, S D Rabkin
Jun 1, 1995·Neuroscience·A P ByrnesH M Charlton
Jun 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·D W PfaffY S Zhu
Dec 25, 2003·Diabetes Research and Clinical Practice·S-i TanakaC Mobbs
Oct 14, 2003·Experimental Neurology·John H RogersStacey Efstathiou
Jan 1, 1997·Journal of Neuroscience Methods·M G Kaplitt, H Makimura
Jan 1, 1997·Journal of Neuroscience Methods·H J FederoffT Corden
Jun 28, 2003·Brain Research. Molecular Brain Research·Roderick E M ScottDonald W Pfaff
Jun 1, 1997·Brain Research. Molecular Brain Research·Z Hannas-DjebbaraB Jacquemont
Jul 8, 1998·Progress in Neurobiology·W T Hermens, J Verhaagen
Oct 20, 1996·Human Gene Therapy·P A PechanX O Breakefield
Feb 10, 1997·Human Gene Therapy·K M JohnstonX O Breakefield
Jun 13, 1998·Journal of Neurotrauma·P A DijkhuizenJ Verhaagen
Oct 6, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·M TodaS D Rabkin
Sep 1, 1995·Stroke; a Journal of Cerebral Circulation·M D LinnikH J Federoff

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