Expression of a functional hyperpolarization-activated current (Ih) in the mouse nucleus reticularis thalami

Journal of Neurophysiology
Y Rateau, N Ropert

Abstract

The GABAergic neurons of the nucleus reticularis thalami (nRT) express the type 2 hyperpolarization-activated cAMP-sensitive (HCN2) subunit mRNA, but surprisingly, they were reported to lack the hyperpolarization-activated (Ih) current carried by this subunit. Using the voltage-clamp recordings in the thalamocortical slice preparation of the newborn and juvenile mice (P6-P23), we demonstrate that, in the presence of 1 mM barium (Ba2+), the nRT neurons express a slow hyperpolarization-activated inward current, suggesting that the Ih is present but masked in control conditions by K+ leak currents. We investigate the identity of the hyperpolarization-activated current in the nRT by studying its physiological and pharmacological profile in presence of Ba2+. We show that it has voltage- and time-dependent properties typical of the Ih, that it is blocked by cesium and ZD7288, two blockers of the Ih, and that it is carried both by the K+ and Na+ ions. We could also alter the gating characteristics of the hyperpolarization-activated current in the nRT by adding a nonhydrolysable analogue of cAMP to the pipette solution. Finally, using the current-clamp recording, we showed that blocking the hyperpolarization-activated current induced a...Continue Reading

References

Oct 28, 1982·Brain Research·J V Halliwell, P R Adams
Aug 1, 1994·Journal of Neurophysiology·A DestexheM Steriade
Oct 29, 1993·Science·M SteriadeT J Sejnowski
Oct 1, 1993·The Journal of Physiology·D ContrerasM Steriade
Aug 1, 1996·Neuron·T Bal, D A McCormick
Jan 1, 1997·Annual Review of Neuroscience·D A McCormick, T Bal
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B SantoroE R Kandel
Jun 26, 1998·Journal of Neurophysiology·A LüthiD A McCormick
Feb 22, 2001·Annual Review of Physiology·U B Kaupp, R Seifert
Dec 14, 2001·The Journal of Biological Chemistry·Catherine ProenzaEric A Accili
Oct 22, 2002·Nature Neuroscience·Andrea LörinczZoltan Nusser
Dec 10, 2002·Annual Review of Physiology·Richard B Robinson, Steven A Siegelbaum
Nov 18, 2003·Nature Reviews. Neuroscience·Delphine BichetLily Yeh Jan
Mar 3, 2004·The Journal of Comparative Neurology·Takuya Notomi, Ryuichi Shigemoto
Apr 20, 2004·The European Journal of Neuroscience·Changqing XuMeenakshi Alreja
Aug 5, 2004·The Journal of Biological Chemistry·Jihong QuRichard B Robinson
Mar 24, 2005·Progress in Neurobiology·Pablo Fuentealba, Mircea Steriade

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Citations

Mar 22, 2013·PLoS Computational Biology·Syed Y AbbasDiana L Pettit
Apr 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeanne T PazJohn R Huguenard
Mar 1, 2014·Pflügers Archiv : European journal of physiology·Vincenzo CrunelliRégis C Lambert
Jun 4, 2014·The European Journal of Neuroscience·Daniel Ulrich
Jun 7, 2008·The Journal of Physiology·Sunggu Yang, Charles L Cox
Aug 11, 2007·The Journal of Physiology·B A GrahamR J Callister
Dec 18, 2008·The European Journal of Neuroscience·Armantas BaginskasMorten Raastad
Jul 9, 2009·Physiological Reviews·Martin BielXiangang Zong
Jan 13, 2016·Anesthesia and Analgesia·Tao HuTao Liu

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