PMID: 18719741Aug 23, 2008Paper

Expression of a hantavirus N protein and its efficacy as antigen in immune assays

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
L T M FigueiredoV H Aquino

Abstract

Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening o...Continue Reading

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Citations

Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Dec 8, 2009·Emerging Infectious Diseases·Jean E LimongiMarcelo S Ferreira
Jun 21, 2015·Expert Review of Anti-infective Therapy·Salim MattarLuis Tadeu Figueiredo
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