Expression of a Lactococcus lactis Phage Resistance Mechanism by Streptococcus thermophilus.

Applied and Environmental Microbiology
S MoineauP A Vandenbergh

Abstract

The 7.8-kb lactococcal plasmid pSRQ700 encodes the LlaII restriction/modification system which recognizes and cleaves the sequence 3(prm1)-GATC-5(prm1). When the plasmid pSRQ700 is introduced into a phage-sensitive Lactococcus lactis strain, strong phage resistance is conferred by the LlaII system. In this report, we show that pSRQ700 cannot replicate in Streptococcus thermophilus. However, if cloned into the vector pNZ123, the native LlaII system is expressed and strong phage resistance is conferred to various industrial S. thermophilus strains. Resistance against phages isolated from yogurt and mozzarella wheys was observed. To our knowledge, this is the first report of increased phage resistance in S. thermophilus.

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Citations

Jul 4, 2013·Nature Communications·Marie-Ève DupuisSylvain Moineau
Jul 27, 2014·Nucleic Acids Research·Bruno Martel, Sylvain Moineau
Feb 2, 2002·Applied and Environmental Microbiology·Joseph M Sturino, Todd R Klaenhammer
Apr 7, 2006·Applied and Environmental Microbiology·David M DonovanDavid G Pritchard
Mar 5, 2005·Applied and Environmental Microbiology·Geneviève LamotheSylvain Moineau
Jul 25, 2014·Nature Communications·Alexander P HynesSylvain Moineau
Nov 27, 2002·Applied and Environmental Microbiology·Mark Tangney, Gerald F Fitzgerald
Nov 29, 2017·Applied and Environmental Microbiology·Brian McDonnellDouwe van Sinderen
Oct 17, 2020·Critical Reviews in Biochemistry and Molecular Biology·Taylor M NyeLyle A Simmons
Jan 9, 2004·Plasmid·Nathalie TurgeonSylvain Moineau
Feb 4, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Cécile Philippe, Sylvain Moineau

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