PMID: 8599190Oct 1, 1995Paper

Expression of a large, nontoxic fragment of botulinum neurotoxin serotype A and its use as an immunogen

Toxicon : Official Journal of the International Society on Toxinology
H F LaPenotiereJohn L Middlebrook

Abstract

Using the polymerase chain reaction, a large fragment of botulinum toxin was placed in two expression systems, one designed to produce a fusion protein product and another designed to produce only the toxin fragment. Expression of the fragment in the latter system was inconsistent. Expression of the fusion protein was easily measurable by ELISA. Mice were vaccinated with crude fusion protein, then challenged with native toxin. Mice receiving two immunizations were partially protected from up to 1200 LD50, suggesting that this toxin fragment may be a good vaccine candidate to replace the currently used toxoid.

References

Mar 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·H Niemann
Jun 1, 1989·Archives of Disease in Childhood·G E SmithR J Gilbert

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Citations

Aug 13, 2011·Bioprocess and Biosystems Engineering·Kheirollah YariMahmood Tavallaei
May 18, 2005·Advanced Drug Delivery Reviews·John L Middlebrook
Oct 29, 1998·Toxicon : Official Journal of the International Society on Toxinology·L A Smith
Nov 22, 2000·Biochimie·M P Byrne, L A Smith
Nov 6, 2010·Protein Engineering, Design & Selection : PEDS·Mengfei HoBrenda A Wilson
Sep 13, 2001·Infection and Immunity·B P MullaneyJ D Marks
Jan 29, 2014·World Journal of Microbiology & Biotechnology·Ebrahim ValipourShahram Nazarian
Apr 24, 2010·Biotechnology and Applied Biochemistry·Kheirollah YariMahmoud Tavallaei
Mar 18, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·M Zouhair Atassi
Jul 24, 2004·Expert Review of Vaccines·Jong-Beak Park, Lance L Simpson
Aug 6, 2004·Protein Expression and Purification·Michael R BaldwinJoseph T Barbieri
Jun 7, 2005·Advanced Drug Delivery Reviews·Vincent W BramwellH Oya Alpar
Sep 5, 2017·Toxins·Grace Sundeen, Joseph T Barbieri
Aug 1, 2008·Clinical and Vaccine Immunology : CVI·James E Keller
Oct 11, 2008·Clinical and Vaccine Immunology : CVI·Yun-Zhou YuZhi-Wei Sun

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