PMID: 9192697Jun 24, 1997Paper

Expression of a mutant alpha-zein creates the floury2 phenotype in transgenic maize

Proceedings of the National Academy of Sciences of the United States of America
C E ColemanBrian A Larkins

Abstract

The maize floury2 mutation results in the formation of a soft, starchy endosperm with a reduced amount of prolamin (zein) proteins and twice the lysine content of the wild type. The mutation is semidominant and is associated with small, irregularly shaped protein bodies, elevated levels of a 70-kDa chaperone in the endoplasmic reticulum, and a novel 24-kDa polypeptide in the zein fraction. The 24-kDa polypeptide is a precursor of a 22-kDa alpha-zein protein that is not properly processed. The defect is due to an alanine-to-valine substitution at the C-terminal position of the signal peptide, which causes the protein to be anchored to the endoplasmic reticulum. We postulated that the phenotype associated with the floury2 mutation is caused by the accumulation of the 24-kDa alpha-zein protein. To test this hypothesis, we created transgenic maize plants that produce the mutant protein. We found that endosperm in seeds of these plants manifests the floury2 phenotype, thereby confirming that the mutant alpha-zein is the molecular basis of this mutation.

References

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Citations

Apr 23, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·David R HoldingBrian A Larkins
Jun 9, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yongrui WuJoachim Messing
Apr 2, 2008·Plant Molecular Biology·Pau MarzábalM Dolores Ludevid
Mar 6, 1999·Current Opinion in Plant Biology·T KomariT Kubo
Mar 6, 1999·Current Opinion in Plant Biology·D R Gallie
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Aug 21, 2013·Nature Communications·Yongrui WuJoachim Messing
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jian-Hong Xu, Joachim Messing
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yongrui WuJoachim Messing
Jan 8, 2009·Plant Physiology·Paolo A Sabelli, Brian A Larkins
Mar 11, 1999·The Plant Cell·N Leborgne-CastelJ Denecke
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rentao Song, Joachim Messing
Sep 30, 2010·Genetics·Yongrui Wu, Joachim Messing
Dec 27, 2015·Plant Science : an International Journal of Experimental Plant Biology·Mustapha BenmoussaBruce R Hamaker
Jul 8, 2014·Frontiers in Plant Science·David R Holding
Feb 5, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·J Beslin JoshiD Sudhakar
Dec 17, 2014·Plant Molecular Biology·H W WongS S M Sun
Apr 15, 2016·PLoS Genetics·Zhenyi QiaoRentao Song
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Cheol Soo KimRudolf Jung
Aug 26, 2009·Plant Biotechnology Journal·Jinrui ZhangMichael J Giroux
Apr 15, 2006·Plant & Cell Physiology·Hiroshi YasudaFumio Takaiwa
Jan 4, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chaobin Li, Rentao Song

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