PMID: 8610137Apr 2, 1996Paper

Expression of a protective gene-prolongs survival of T cells in human immunodeficiency virus-infected patients

Proceedings of the National Academy of Sciences of the United States of America
C WoffendinGary J Nabel

Abstract

The resistance of acquired immunodeficiency syndrome (AIDS) to traditional drug therapy has prompted a search for alternative treatments for this disease. One potential approach is to provide genetic resistance to viral replication to prolong latency. This strategy requires the definition of effective antiviral genes that extend the survival of T cells in human immunodeficiency virus (HIV)-infected individuals. We report the results of a human study designed to determine whether a genetic intervention can prolong the survival of T cells in HIV-infected individuals. Gene transfer was performed in enriched CD4+ cells with plasmid expression vectors encoding an inhibitory Rev protein, Rev M10, or a deletion mutant control, deltaRev M10, delivered by gold microparticles. Autologous cells separately transfected with each of the vectors were returned to each patient, and toxicity, gene expression, and survival of genetically modified cells were assessed. Cells that expressed Rev M10 were more resistant to HIV infection than those with deltaRev M10 in vitro. In HIV-infected subjects, Rev M10-transduced cells showed preferential survival compared to deltaRev M10 controls. Rev M10 can therefore act as a specific intracellular inhibitor ...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D BevecE Böhnlein
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J O OjwangJ Rappaport
Jan 1, 1991·Annual Review of Biochemistry·Y N Vaishnav, F Wong-Staal
Mar 9, 1990·Science·N SarverJ J Rossi
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L PearsonR Gaynor
Sep 29, 1988·Nature·D Baltimore
May 1, 1993·Antimicrobial Agents and Chemotherapy·T J DuewekeL Resnick
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·C WoffendinG J Nabel
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L DuanR J Pomerantz
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M YuF Wong-Staal
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W A MarascoS Y Chen
Aug 11, 1993·Nucleic Acids Research· UdaykumarI K Hewlett

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Citations

Dec 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Bank
Aug 26, 2010·Immunologic Research·Geoff P SymondsLouis R Breton
Jun 7, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mi Ran ParkChong Su Cho
Sep 13, 2003·Fertility and Sterility·Gaurang S Daftary, Hugh S Taylor
Mar 10, 2000·Biochimica Et Biophysica Acta·S ToddC S Craik
Dec 1, 1996·Trends in Genetics : TIG·L A Couture, D T Stinchcomb
May 7, 1997·Transfusion Science·T Sorg, M Methali
May 7, 1997·Transfusion Science·B Péault, P Charbord
Sep 28, 1999·Molecular Medicine Today·R A Morgan
Mar 16, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jan van LunzenDorothee von Laer
Dec 11, 2007·Nature Biotechnology·John J RossiDonald B Kohn
Dec 11, 2007·Nature Biotechnology·Joost HaasnootBen Berkhout
Apr 1, 1997·Nature Medicine·R H Miller, N Sarver
Dec 12, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ananthalakshmi Poluri, Richard E Sutton
Jun 12, 2001·Human Gene Therapy·G L Buchschacher, F Wong-Staal
Feb 13, 2002·AIDS Patient Care and STDs·Mario R Mautino, Richard A Morgan
Nov 25, 2000·Journal of Hematotherapy & Stem Cell Research·Y HanazonoC E Dunbar
Nov 14, 1997·AIDS Research and Human Retroviruses·U JunkerE Böhnlein
Nov 21, 1998·AIDS Research and Human Retroviruses·O S LundJ E Hansen
May 22, 2010·Human Gene Therapy·Bianca HeemskerkTon N M Schumacher
Jul 7, 2007·Journal of Biomedical Optics·Jesse AaronKonstantin Sokolov
Jul 16, 2008·Antimicrobial Agents and Chemotherapy·Deidra Shuck-LeeDavid Rekosh
Dec 25, 2003·Journal of Virology·Marc EgelhoferDorothee von Laer

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