Expression of a recombinant bifunctional protein from a chimera of human lutropin receptor and human chorionic gonadotropin beta-subunit

Journal of Reproductive Immunology
Meirong HaoBrij Saxena

Abstract

Human lutropin (hLH) and human chorionic gonadotropin (hCG) are structurally and functionally similar and play important roles in reproduction via a common gonadal receptor (LH-R). However, hormone specific hCG-beta subunit contains 24 additional amino acid carboxyl terminal peptide (CTP), which produce specific antibodies to hCG-beta with little cross-reaction with LH. A chimeric protein containing both hLH-R and hCG-beta would provide a unique bifunctional antigen for immunocontraception. In this study is described the synthesis of a chimeric DNA construct of full-length of hLH-receptor and hCG-beta and its expression in Sf9 cells to produce a bifunctional protein. Recombinant protein was recognized by antibodies to LH-R as well as anti-hCG-beta in Western Blots, thus indicating the preservation of immunological epitopes for both hLH-R and hCG-beta in the chimera. Specific ligand binding of recombinant hLH-R component was demonstrated by the displacement of bound labeled hCG at increasing concentrations of unlabeled hCG, indicating that, the presence of hCG-beta component of the chimera did not interfere with the binding of hCG to LH-R. hCG-beta was also present in the recombinant chimeric protein as shown by a specific hCG-b...Continue Reading

References

Dec 1, 1977·American Journal of Obstetrics and Gynecology·G T Ross
Jun 1, 1992·Journal of Reproductive Immunology·R PalB B Saxena
Mar 1, 1992·Journal of Reproductive Immunology·R PalB B Saxena
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R K CampbellW R Moyle
Jan 16, 1985·Biochemical and Biophysical Research Communications·L A ColeF Perini
Jul 15, 1972·American Journal of Obstetrics and Gynecology·J L VaitukaitisG T Ross
Feb 1, 1995·Journal of Immunoassay·M SinghB B Saxena
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T SugaharaI Boime
Dec 6, 1996·The Journal of Biological Chemistry·C WuD Puett
May 1, 1997·European Journal of Biochemistry·J C HeikoopP D Grootenhuis
Aug 26, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T PaukkuI T Huhtaniemi
Mar 8, 2002·Reproduction in Domestic Animals = Zuchthygiene·B B SaxenaScott Perkins
Apr 12, 2002·Endocrine Reviews·Mario AscoliDeborah L Segaloff
Mar 29, 2003·American Journal of Veterinary Research·Brij B SaxenaScott Perkins

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