Expression of a soluble TGF-beta receptor by tumor cells enhances dendritic cell/tumor fusion vaccine efficacy

The Journal of Immunology : Official Journal of the American Association of Immunologists
Min ZhangJohn Y Kao

Abstract

Dendritic cell (DC)-based antitumor immunotherapy is a promising cancer therapy. We have previously shown that tumor-derived TGF-beta limits the efficacy of the DC/tumor fusion vaccine in mice. In the current study we investigated the effect of neutralizing tumor-derived TGF-beta on the efficacy of the DC/tumor fusion vaccine. An adenovirus encoding human TGF-beta receptor type II fused to the Fc region of human IgM (Adv-TGF-beta-R) or a control adenovirus encoding LacZ (Adv-LacZ) was used to express a soluble form of the neutralizing TGF-beta receptor (TGF-beta-R). Murine breast carcinoma cells, 4T1, but not bone marrow-derived DCs, were successfully transfected with Adv-TGF-beta-R (4T1+Adv-TGF-beta-R) using a multiplicity of infection of 300. Immunization with irradiated 4T1+Adv-TGF-beta-R tumor cells conferred enhanced antitumor immunity compared with immunization with irradiated 4T1+Adv-LacZ tumor cells. The DC/4T1+Adv-TGF-beta-R fusion vaccine offered enhanced protective and therapeutic efficacy compared with the DC/4T1-Adv-LacZ fusion vaccine. Because TGF-beta is known to induce regulatory T cells (Tregs), we further showed that the DC/4T1+Adv-TGF-beta-R fusion vaccine induced fewer CD4(+)CD25(+)Foxp3(+) Tregs than the DC...Continue Reading

References

Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Jan 10, 2001·International Journal of Cancer. Journal International Du Cancer·J A McEarchernE T Akporiaye
Feb 24, 2001·CA: a Cancer Journal for Clinicians·B A TjoaG P Murphy
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John Y KaoJian-Jun Chen
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alexander V PereboevJerry L Blackwell
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fahao ZhangZhongyun Dong
May 11, 2006·Blood·Sergio RutellaGiuseppe Leone
Sep 16, 2006·Immunological Reviews·Sharon M WahlNiki Moutsopoulos
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael P SeilerBrendan Lee
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ilona KryczekWeiping Zou
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Teruo KaigaHideaki Tahara
Oct 27, 2007·Taiwanese Journal of Obstetrics & Gynecology·Chi-Mou JuangMing-Shien Yen

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Citations

May 9, 2012·Expert Opinion on Biological Therapy·Keith A Lawson, Don G Morris
May 31, 2016·International Journal of Molecular Sciences·Shigeo Koido
Apr 10, 2010·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Feb 26, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Jan 1, 2009·Immunotherapy·Shigeo KoidoHisao Tajiri
May 18, 2016·World Journal of Gastroenterology : WJG·Mikio KajiharaShigeo Koido
May 5, 2011·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Oct 20, 2010·Expert Opinion on Biological Therapy·Michael R ShurinAnatoli M Malyguine
Feb 7, 2017·Nature Communications·Nirit Mor-VakninDavid M Markovitz
Jun 3, 2017·Human Vaccines & Immunotherapeutics·Sulsal Haque, John C Morris
Nov 5, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Dec 17, 2019·International Immunopharmacology·Vida HashemiFarhad Jadidi-Niaragh
Mar 17, 2021·International Immunopharmacology·Kanan PanchalAkash Chaurasiya
Sep 13, 2011·Cancer Cell·Zvi GranotRobert Benezra

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