Abstract
When mRNA for avian retroviral envolope glycoprotein (env) was injected into cells transformed by env-deficient Bryan Rous sarcoma virus, the env deficiency of the injected cells was complemented to allow the release of transforming virus for up to 40 hr. When virus spread within the injected culture was allowed to occur, a second phase of transforming virus production by the injected culture began approximately 2 days following injection, continued for many days and often increased to titers well above those seen soon after injection. The requirement for virus spread, along with the genetic properties of virus released long after injection, supported the hypothesis that the second phase of virus production resulted when injected env mRNA was packaged into virus released by injected cells. When this virus infected other cells within the culture the env mRNA was reverse-transcribed to form a subgenomic, proviral-like molecule able to direct the synthesis of env mRNA. Accordingly, it was shown tht neither DNA nor full genomic viral RNA contaminating injected mRNA preparations could account for the results. Evidence that an mRNA can be reverse-transcribed into an active, proviral-like molecule may be of importance in the relations...Continue Reading
Citations
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J J KopchickD W Stacey
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A W Stoker, M H Sieweke
Apr 1, 1994·Human Gene Therapy·J A Wolff, J Lederberg
Oct 11, 1982·Nucleic Acids Research·B R CullenD W Stacey
Jul 1, 1987·European Journal of Biochemistry·E S Kuczek, G E Rogers
Jan 15, 2005·Journal of Biomaterials Science. Polymer Edition·Seog-Jin SeoChong-Su Cho
Sep 1, 1992·Virology·O HantzC Trepo
Mar 1, 1982·Virology·W S MasonJ M Taylor
Oct 1, 1981·Cell·K Shimotohno, H M Temin
Oct 1, 1987·Journal of Virology·M NishizawaS Kawai
Mar 1, 1986·Journal of Virology·P MartinS Saule
Aug 1, 1986·Journal of Virology·T PatschinskyK Bister
Oct 1, 1991·Journal of Virology·S TerréC Bréchot
May 1, 1984·Journal of Virology·D L Robertson
Feb 1, 1989·Journal of Virology·J Bodor, J Svoboda
Apr 1, 1984·Journal of Virology·J ChinskyJ Kopchick
Sep 1, 1983·Journal of Virology·J E Majors, H E Varmus
Jul 1, 1984·Journal of Virology·S Kawai, T Koyama
Mar 1, 1982·Journal of Virology·L H Wang, D W Stacey
Jul 1, 1984·Journal of Virology·T KoyamaS Kawai
Aug 1, 1982·Journal of Virology·T Pugatsch, D W Stacey