Expression of ABCA3, a causative gene for fatal surfactant deficiency, is up-regulated by glucocorticoids in lung alveolar type II cells

Biochemical and Biophysical Research Communications
Ichiro YoshidaNobuya Inagaki

Abstract

We have shown previously that the ATP-binding cassette transporter ABCA3 is expressed predominantly at the limiting membrane of the lamellar bodies in lung alveolar type II cells. Very recently, an ABCA3 gene mutation was reported in human newborns with fatal surfactant deficiency. In the present study, we have shown in rat lung that expression of the ABCA3 protein is dramatically increased after embryonic day (E) 20.5 just before birth. Expression was also markedly induced even at E18.5 when dexamethasone (Dex), which is known to accelerate surfactant formation, was administered to pregnant female rats for 3 days from E15.5. Since Dex increased the ABCA3 mRNA expression level in human alveolar type II cell line A549 cells 4-fold, we cloned and characterized the promoter region of the human ABCA3 gene. Promoter activity of the 5'-flanking region of the ABCA3 gene, which contains a potential glucocorticoid-responsive element (GRE), was up-regulated about 2-fold. Up-regulation by Dex was not observed when the GRE-containing region was deleted or when a point mutation was introduced into the GRE, and electrophoretic mobility shift assay using Dex-treated A549 nuclear extracts demonstrated specific binding of the glucocorticoid rec...Continue Reading

References

May 1, 1978·Endocrinology·P L BallardW H Douglas
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·V C Venkatesh, P L Ballard
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·D E Schellhase, J M Shannon
Sep 1, 1989·Pediatric Research·D E SchellhaseJ M Shannon
May 1, 1989·Endocrine Reviews·P L Ballard
Dec 1, 1969·The Journal of Endocrinology·G C Liggins
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J C ClarkJ A Whitsett
Jun 1, 1994·The Australian & New Zealand Journal of Obstetrics & Gynaecology·G C Liggins
Jul 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M T StahlmanJ A Whitsett
Mar 15, 1997·European Journal of Biochemistry·J Johansson, T Curstedt
Feb 21, 1998·Nucleic Acids Research·T HeinemeyerN A Kolchanov
Dec 3, 1999·Biochimica Et Biophysica Acta·C BroccardoG Chimini
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·E CutzA M Moore
Sep 6, 2000·American Journal of Human Genetics·R Allikmets
Jun 21, 2001·Pediatric Pulmonology·R J BoltH A Delemarre-van de Waal
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Janne HukkanenJukka Hakkola
Mar 27, 2004·The New England Journal of Medicine·Sergey ShuleninMichael Dean

❮ Previous
Next ❯

Citations

Apr 6, 2006·Pflügers Archiv : European journal of physiology·Christiane Albrecht, Enrique Viturro
Mar 1, 2010·Pediatric Allergy, Immunology, and Pulmonology·Lawrence M Nogee
Jul 18, 2008·Human Molecular Genetics·Teruki YanagiHiroshi Shimizu
Mar 2, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Valérie BesnardJeffrey A Whitsett
Sep 9, 2006·American Journal of Respiratory Cell and Molecular Biology·Venkatadri KollaPhilip L Ballard
Dec 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Yohei MatsuzakiJeffrey A Whitsett
Aug 24, 2010·Orphanet Journal of Rare Diseases·Annick ClementHarriet Corvol
Jun 19, 2007·FEBS Letters·Yoshihiro MatsumuraNobuya Inagaki
Dec 5, 2006·Seminars in Perinatology·Janine E BullardLawrence M Nogee
Oct 10, 2006·Pediatric Clinics of North America·Jeffrey A Whitsett, Yohei Matsuzaki
Oct 29, 2015·Pediatrics International : Official Journal of the Japan Pediatric Society·Fiammetta PiersigilliAndrea Dotta
Jul 3, 2013·Pediatric Pulmonology·Surendran ThavagnanamSharon D Dell
Feb 15, 2014·Biochimica Et Biophysica Acta·Elena Lopez-Rodriguez, Jesús Pérez-Gil
Mar 17, 2006·Biochimica Et Biophysica Acta·Wolfgang E KaminskiJürgen J Wenzel
Jul 16, 2016·Archives of Disease in Childhood·Atul Gupta, Sean Lee Zheng
Sep 20, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mildred T StahlmanJeffrey A Whitsett
Dec 24, 2014·Molecular Endocrinology·A Daniel BirdTimothy J Cole
Feb 3, 2007·The Journal of Biological Chemistry·Nobuhiro BanNobuya Inagaki
May 10, 2007·The Journal of Biological Chemistry·Hiromichi SakaiNobuya Inagaki
Feb 5, 2008·Annals of Medicine·Minna K KarjalainenMikko Hallman
Sep 25, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Valérie BesnardJeffrey A Whitsett
Mar 1, 2017·Respiratory Research·Amanda B ChaiIngrid C Gelissen
Sep 9, 2006·The Journal of Biological Chemistry·Yoshihiro MatsumuraNobuya Inagaki
Nov 1, 2015·Biochemical Society Transactions·Donatella PecaOlivier Danhaive
Mar 6, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wolfgang BernhardChristian F Poets
Aug 5, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yoshihiro MatsumuraNobuya Inagaki
Feb 3, 2021·Journal of Medical Case Reports·Waleed ShaabanNawal Alkazemi
May 6, 2021·International Journal of Molecular Sciences·Narakorn Khunweeraphong, Karl Kuchler
Jun 5, 2021·Environmental Science and Pollution Research International·Tapan BehlSimona Bungau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The New England Journal of Medicine
Sergey ShuleninMichael Dean
The Journal of Biological Chemistry
Nobuhiro BanNobuya Inagaki
American Journal of Respiratory and Critical Care Medicine
Janine E BullardL M Nogee
The New England Journal of Medicine
J A Whitsett, Timothy E Weaver
© 2021 Meta ULC. All rights reserved