Expression of aberrantly glycosylated tumor mucin-1 on human DC after transduction with a fiber-modified adenoviral vector

Cytotherapy
E B M van LeeuwenGerard M J Bos

Abstract

DC-presenting tumor Ag are currently being developed to be used as a vaccine in human cancer immunotherapy. To increase chances for successful therapy it is important to deliver full-length tumor Ag instead of loading single peptides. In this study we used a fiber-modified adenoviral vector (rAd5F35) containing full-length tumor Ag cDNA to transduce human monocyte (Mo)-derived DC in vitro. Cells were efficiently transduced and survived for at least 3 days after adenoviral transduction. Phenotype and function after maturation of Mo-DC were not impaired by infection with adenovirus particles. Expression of the tumor-associated Ag mucin-1 (MUC1) was detected using MAb defining different MUC1 glycoforms. Non-transduced mature Mo-DC express endogenous MUC1 with normal glycosylation. After transduction with the rAd5F35-MUC1 adenoviral vector, Mo-DC also expressed MUC1 with tumor-associated glycosylation (Tn and T glycoforms), although no changes in mRNA levels of relevant glycosyltransferases could be demonstrated. The presence of aberrantly glycosylated MUC1 may influence Ag presentation of the tumor glycoforms of MUC1 to immune cells, affecting tumor cell killing. These findings could be highly relevant to developing strategies for...Continue Reading

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Citations

Sep 5, 2008·Expert Review of Vaccines·Choon-Kit TangVasso Apostolopoulos
Nov 9, 2007·Biochimica Et Biophysica Acta·Mads Agervig Tarp, Henrik Clausen
Oct 3, 2006·British Journal of Haematology·Silvie CloosenGerard M J Bos
Oct 20, 2010·Histopathology·Catharina H M J Van ElssenGerard M J Bos
Jan 26, 2007·Expert Opinion on Biological Therapy·Robert W MaysRobert Deans
Jan 5, 2011·Journal of Immunological Methods·Catharina H M J Van ElssenJoris Vanderlocht
Aug 7, 2008·Virchows Archiv : an International Journal of Pathology·Svend KirkebySteen Seier Poulsen

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