Expression of Acc-Royalisin gene from royal jelly of Chinese honeybee in Escherichia coli and its antibacterial activity

Journal of Agricultural and Food Chemistry
Lirong ShenDuo Li

Abstract

Royalisin is an antibacterial peptide found in Royal Jelly. Two gene fragments of Chinese honeybee (Apis cerana cerana) head, 280 bp cDNA encoding pre-pro-Acc-royalisin (PPAR) of 95 amino acid residues, and 165 bp cDNA encoding mature Acc-royalisin (MAR) of 51 amino acid residues were cloned into the pGEX-4T-2 vector. They were then transformed individually into Escherichia coli for expression. Two expressed fusion proteins, glutathione S-transferase (GST)-PPAR of 36 kDa and GST-MAR of 32 kDa were obtained, which were cross reacted with GST antibody accounting for up to 16.3% and 15.4% of bacterial protein, respectively. In addition, 41% of GST-PPAR and nearly 100% of GST-MAR were soluble proteins. Both lysates of the two purified fusion proteins displayed antibacterial activities, similar to that of nisin against Gram-positive bacteria strains, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus. MAR peptide released from the thrombin-cleaved GST-MAR fusion protein has a stronger antibacterial activity than that of GST-MAR fusion protein.

References

Feb 1, 1996·Antonie van Leeuwenhoek·J Delves-BroughtonJ Hugenholtz
Nov 9, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·L Otvos
Jan 5, 2002·International Journal of Food Microbiology·J ClevelandM L Chikindas
Dec 21, 2004·Insect Biochemistry and Molecular Biology·Jaroslav KlaudinyJozef Simúth
Oct 31, 2006·Nature·UNKNOWN Honeybee Genome Sequencing Consortium
Oct 12, 2007·Journal of Agricultural and Food Chemistry·Jianke LiYinghong Pan
Apr 29, 2009·PloS One·Franziska B MullauerJan Paul Medema

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Citations

Feb 11, 2016·Journal of Medical Microbiology·Martin SojkaJuraj Majtan
Feb 28, 2013·Food Chemistry·Reo NozakiToru Kono
Sep 25, 2016·Microbiological Research·Filippo FratiniAntonio Felicioli
Jul 9, 2016·Journal of Agricultural and Food Chemistry·Xiao-Xuan XinLi-Rong Shen
Sep 2, 2016·Journal of Biomolecular Structure & Dynamics·Marco FranzoiStefano Mammi

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