Expression of activin A in human keratinocytes at early stages of cultivation

FEBS Letters
M SeishimaY Kitajima

Abstract

Activins are members of the TGF-beta superfamily and are classified into 3 types: activin A, which consists of a homodimer of betaA, activin B, which consists of a homodimer of betaB, and activin AB, which consists of a heterodimer of betaAbetaB. We studied the expression of activin mRNAs by RT-PCR in normal human epidermis, cultured keratinocytes, and DJM-1 cells (a squamous cell carcinoma line). We could detect only activin A mRNA (betaA) in normal human epidermis. In cultured keratinocytes and DJM-1 cells, activin betaA mRNA was observed at 4 h but not at 96 h after plating. Activin A activity was detected in the conditioned medium of DJM-1 cells within 48 h. In addition, although follistatin mRNA was not observed in human epidermis in situ, it was transiently expressed in cultured cells at 4 h after plating. These findings suggest that the expression of these molecules in keratinocytes is associated with cell proliferation. In an in vitro tissue injury model, activin A was observed at the wound edge, where cell migration and proliferation may be activated. In DJM-1 cells cultured for 92 h, betaA mRNA was observed 4 h after injury treatment. These findings suggest that activin A acts as a potent inducer of proliferation in v...Continue Reading

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Citations

Feb 13, 2001·Molecular and Cellular Endocrinology·S KawakamiS J Winters
May 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A ShimizuK Miyazono
Aug 28, 2009·The Journal of Investigative Dermatology·Janna NousbeckEli Sprecher
Apr 14, 1999·The Journal of Investigative Dermatology·M SeishimaY Kitajima
Sep 27, 2005·European Journal of Cell Biology·Patrizia StoitznerNikolaus Romani
Mar 25, 2004·The American Journal of Pathology·Vassiliki PoulakiAntonia M Joussen

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