Expression of activity-dependent neuroprotective protein in the immune system: possible functions and relevance to multiple sclerosis.

Neuroimmunomodulation
Manjit BraitchCris S Constantinescu

Abstract

Activity-dependent neuroprotector (ADNP) is a neuroprotective molecule containing an 8-amino acid peptide, NAPVSIPQ (NAP), that is sufficient for its neuroprotective effects. To assess the expression of ADNP in the human immune system in normal subjects and multiple sclerosis patients. MaterialsandMethods: ADNP expression was assessed in peripheral blood mononuclear cells (PBMCs) from healthy donors and multiple sclerosis (MS) patients using staining with anti-ADNP (NAP) antibodies and markers for T cells, B cells, monocytes and natural killer cells. ADNP mRNA was determined in peripheral blood from MS patients (n = 24) and matched controls (n = 21). Expression of activation markers CD69 and CD154 and of IFN-gamma was assessed by flow cytometry in stimulated PBMCs. Effects of NAP on immune cell proliferation was assessed by tritiated thymidine incorporation. Monocytes, B cells and T cells, but not regulatory (CD4+CD25+) T cells expressed ADNP. NAP peptide decreased the expression of CD69, CD154 and IFN-gamma in PBMC and caused suppressed anti-CD3-/anti-CD28-stimulated PBMC proliferation. ADNP mRNA was reduced in MS compared to control peripheral blood. ADNP is expressed in many immune system cells. ADNP mRNA is reduced in PBMCs...Continue Reading

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Citations

Mar 5, 2011·British Journal of Pharmacology·Cris S ConstantinescuBruno Gran
Dec 11, 2013·Brain, Behavior, and Immunity·Marta PedreñoElena Gonzalez-Rey
Aug 15, 2018·The Journal of Clinical Investigation·Gal Hacohen-KleimanIllana Gozes
Dec 13, 2017·Oncotarget·José M García-Heredia, Amancio Carnero
Jul 13, 2018·European Journal of Microbiology & Immunology·Ulrike EscherMarkus M Heimesaat
Sep 20, 2019·Translational Psychiatry·Shlomo SragovichIllana Gozes
Nov 19, 2015·Frontiers in Aging Neuroscience·Illana Gozes, Yanina Ivashko-Pachima
Sep 1, 2015·Journal of Molecular Neuroscience : MN·Anna MalishkevichIllana Gozes
Aug 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luciana Souza-MoreiraElena Gonzalez-Rey
Jun 15, 2018·European Journal of Human Genetics : EJHG·Minh-Tuan HuynhCatherine Vincent-Delorme

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Methods Mentioned

BETA
FCS
PCR
flow cytometry

Software Mentioned

WinMDI

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