Expression of adhesion molecules in cultured human pulmonary microvascular endothelial cells

Microvascular Research
J ShenS Karmiol

Abstract

HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the presence of factor VIII-related antigen, incorporation of DiI-Ac-LDL, tubule-like structure formation in Matrigel, and positive staining for ACE. Adhesion molecules were tested at passage 3 and passage 12. Cells demonstrated intense staining for PECAM-1 both unstimulated and stimulated with TNF-alpha (20 ng/ml). The adhesion molecules ICAM-1, VCAM-1, ELAM-1, and P-selectin differed in expression on unstimulated cells. ICAM-1 was constitutively expressed on unstimulated cells and the expression was increased by TNF-alpha stimulation (20 hr). In contrast, VCAM-1, ELAM-1, and P-selectin were not detected on unstimulated cells but were detected after stimulation with TNF-alpha. The inducibility of adhesion molecules was different. VCAM-1 (10 hr) and ELAM-1 (4 hr) were expressed more strongly than P-selectin (minutes to 4 hr). The adhesion molecule profile found on passage 12 was the same as on passage 3. CD36 was not detec...Continue Reading

Citations

Apr 12, 2011·The Journal of Biological Chemistry·Nicola Lo BuonoAda Funaro
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·A Burke-Gaffney, P G Hellewell
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·H YamamotoW W Busse
May 20, 2009·The Journal of Clinical Investigation·Akinori HoboKenji Kadomatsu
Jun 24, 2011·PloS One·Magomed KhaidakovJawahar L Mehta
Aug 23, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L ThomsonG M Walsh
Feb 18, 2010·International Journal of Cancer. Journal International Du Cancer·Gangadharan B SajithlalEdward V Prochownik
Jun 4, 2013·Basic & Clinical Pharmacology & Toxicology·Anders G KjaergaardLise Wogensen
Aug 10, 2000·Differentiation; Research in Biological Diversity·B Fernandez PujolK Havemann
Sep 1, 1996·Circulation Research·M J EppihimerD N Granger
Jan 3, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Naoki MiyaoKazuhiro Yamaguchi
Feb 1, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hira RaheelWarren L Lee
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q Wang, C M Doerschuk
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q Wang, C M Doerschuk
Jun 21, 2001·Endothelium : Journal of Endothelial Cell Research·R M McDouallS P Allen
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kaushik ParthasarathiJahar Bhattacharya
May 16, 2002·Experimental and Molecular Pathology·Annette M MüllerC James Kirkpatrick

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