PMID: 11910588Mar 26, 2002Paper

Expression of adhesion molecules LFA-I and ICAM-I on osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency

Climacteric : the Journal of the International Menopause Society
Y GaoT Aso

Abstract

Estrogen deficiency caused by the menopause or ovariectomy leads to stimulation of osteoclastogenesis. The adhesion molecules, leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1), are necessary for osteoclast formation. In this study, the expression of LFA-1 and ICAM-1 on osteoclast precursors during osteoclast differentiation, and the involvement of ovariectomy in the expression, were investigated. Spleen cells isolated from normal or ovariectomized (OVX) mice were co-cultured with TMS14, stromal cells derived from mouse bone marrow, in the absence or presence of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) for 7 days. On days 3, 5 and 7 of culture, the expression of LFA-1 and ICAM-1 on osteoclast precursors was quantitated using indirect immunofluorescence and confocal laser cytometry, and, on day 7, the number of formed osteoclasts was measured by tartrate-resistant acid phosphatase (TRAP) stain. The level of ICAM-1 expression on osteoclast precursors gradually increased with osteoclast differentiation, whereas that of LFA-1 did not change. A high level of ICAM-1 was observed on the integrin beta 3-positive mononuclear cells. On the osteoclast precursors isolated from OVX mice...Continue Reading

References

Jun 1, 1990·Scandinavian Journal of Immunology·S GulshanW H Stimson
Oct 1, 1990·Proceedings of the Society for Experimental Biology and Medicine·D N Kalu
Jan 1, 1989·Advances in Immunology·T K KishimotoT A Springer
Mar 1, 1985·Annals of Internal Medicine·B EttingerC E Cann
Jul 1, 1986·Acta Endocrinologica·J J WeustenJ H Thijssen
Sep 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C MiyauraY Ohsugi
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G FiorelliM L Brandi
Sep 13, 1993·Biochimica Et Biophysica Acta·T KurachiS Murota
Feb 2, 1995·The New England Journal of Medicine·S C Manolagas, R L Jilka
Jan 1, 1994·Bioelectromagnetics·W T Kaune, D A Savitz
Jul 1, 1996·The Journal of Clinical Investigation·T Caulin-GlaserJ R Bender
Aug 1, 1996·Endocrine Reviews·G D Roodman
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Pacifici
Jun 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SudaN Takahashi
Aug 18, 1997·The Journal of Experimental Medicine·T KamedaM Kumegawa
Oct 23, 1997·The Journal of Clinical Investigation·S C LinS C Manolagas
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H YasudaT Suda
Mar 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE

❮ Previous
Next ❯

Citations

Apr 19, 2005·Expert Opinion on Biological Therapy·Patrick LavigneJulio C Fernandes
Jun 9, 2009·Biochemical and Biophysical Research Communications·Veerle BloemenVincent Everts
Apr 20, 2006·The Journal of Steroid Biochemistry and Molecular Biology·D SaintierM E Cohen-Solal
Jun 7, 2003·Menopause : the Journal of the North American Menopause Society·Christian EgarterJohannes Huber
Feb 15, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kristine M ErlandsonGrace A McComsey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.