Expression of alpha-fetoprotein receptors by human T-lymphocytes during blastic transformation.

Molecular Immunology
J M TorresJ Uriel

Abstract

Alpha-fetoprotein (AFP) and transferrin (Tf) are actively internalized by many growing cells during ontogenic and neoplastic development, including human malignant T- and B-lymphoblastoid cells. Their internalization is, on the contrary, greatly diminished or absent in mature, non-proliferating elements. In the present work, peripheral blood mononuclear cells (PBMCs) and T-lymphocytes, harvested from normal human donors, were induced to blastic transformation with phytohemagglutinin (PHA) and their ability to uptake AFP and Tf was measured and compared with Tf uptake in the same conditions. The capacity of the cells to internalize both proteins was quantified by fluorescence activated cell sorter (FACS) using fluoresceinated derivatives of these proteins. The results obtained show a significant uptake of AFP by T-lymphocytes upon PHA stimulation. The values of AFP incorporation were similar for all the cells studied (PBMCs, T-cells and T4, T8 cell subsets). The time course of AFP uptake paralleled, under the same conditions, the uptake of Tf and the expression of IL2 receptors. AFP uptake increased rapidly from the zero time (resting T-cells) and reached a maximum around 72 hr after PHA activation. Scatchard analysis of kinetic...Continue Reading

References

Sep 15, 1987·International Journal of Cancer. Journal International Du Cancer·J LabordaJ Uriel
Feb 28, 1989·Biochemical and Biophysical Research Communications·C Lafarge-FrayssinetJ Uriel
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J NavalJ Uriel
Mar 1, 1986·The Journal of Clinical Investigation·J B LumB H Bowman
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C ReedR G Hoover
Aug 16, 1984·Biochemical and Biophysical Research Communications·M J VillacampaJ Uriel
Mar 1, 1984·Immunology Today·K N Traill, G Wick

❮ Previous
Next ❯

Citations

Dec 2, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gerald J Mizejewski
Jun 15, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·A A Terentiev, N T Moldogazieva
Apr 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L Sánchez Palazón, A Rodríguez-Burgos
Sep 27, 2000·Biochimica Et Biophysica Acta·J R Gillespie, V N Uversky
Sep 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philippe GabantJosiane Szpirer
Feb 24, 2006·Biochemistry. Biokhimii︠a︡·A A Terentiev, N T Moldogazieva
Oct 10, 2007·Biochemistry. Biokhimii︠a︡·A A Terentiev, N T Moldogazieva
Nov 16, 2013·Biochemistry. Biokhimii︠a︡·N G YabbarovE S Severin
Jun 25, 2008·Annual Review of Biophysics·Vladimir N UverskyA Keith Dunker
Oct 1, 1992·The Journal of Clinical Investigation·Y SuzukiE Alpert
Jan 25, 2013·Bioorganicheskaia khimiia·E S ZubkovaM Iu Men'shikov
May 14, 2004·The Journal of Peptide Research : Official Journal of the American Peptide Society·L A DeFreestJ A Bennett
Jul 1, 1993·Immunological Investigations·M Chakraborty, C Mandal
Feb 26, 2008·Expert Opinion on Biological Therapy·Viktoria N Evdokimova, Lisa H Butterfield
Oct 11, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Janneta TcherkassovaHelen Treshalina
Dec 31, 2002·Expert Review of Anticancer Therapy·Gerald J Mizejewski
Mar 18, 2006·The American Journal of Gastroenterology·Fabio FarinatiFranco Trevisani
Mar 1, 1992·Journal of Cellular Physiology·J M TorresJ Uriel
May 6, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Minyue DongHefeng Huang
Sep 8, 2019·Bulletin of Experimental Biology and Medicine·S A ZamorinaV A Chereshnev
Jan 2, 1991·International Journal of Cancer. Journal International Du Cancer·J M TorresJ Uriel
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Bo LinMengsen Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.