PMID: 7537516May 1, 1995Paper

Expression of alpha-GalNAc glycoproteins by breast cancers

British Journal of Cancer
S A Brooks, A J Leathem

Abstract

The expression of complex carbohydrates recognised by Helix pomatia lectin (HPA, nominal monosaccharide binding specificity alpha-GalNAc) has been shown to predict unfavourable prognosis in breast and other cancers. It has been suggested that the prognostic significance of HPA binding may be through recognition of either Tn epitope (alpha-GalNAc-O-serine/threonine) or blood group A antigen (terminal alpha-1-->3GalNAc attached to the basic H-antigen, Fuc-alpha-1-->2-Gal-beta-1-->4(or 3) GlcNAc-->R). In this study, the expression of glycoproteins terminating in alpha-GalNAc residues was investigated immunohistochemically using HPA and two monoclonal antibodies--BRIC 66 (anti-alpha-GalNAc) and BRIC 111 (anti-Tn). In paraffin sections, 74/87 (85%) of breast cancers expressed HPA-binding ligands, while 28/87 (32%) were positive for BRIC 66 binding and 25/87 (29%) expressed Tn. Distribution of staining patterns were distinctive and different with the three markers. BRIC 66, BRIC 111 and HPA binding to glycoproteins derived from breast cancer homogenates and to blood group A and Tn positive glycoproteins in Western blots confirmed the immunohistochemistry data. The results suggest that the prognostic significance of HPA binding in bre...Continue Reading

Citations

Mar 3, 1999·Applied Biochemistry and Biotechnology·E I BeltrãoL C Coelho
Jul 28, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Eiko AoyanagiShinya Tanaka
Feb 4, 2016·Proteomics·Parvez SyedUrpo Lamminmäki
Jul 22, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuliang JiangTongzhong Ju
Dec 8, 1998·International Journal of Cancer. Journal International Du Cancer·P J BostickD S Hoon
Feb 18, 1998·International Journal of Cancer. Journal International Du Cancer·P D RyeM Bryne
Oct 28, 2005·Chembiochem : a European Journal of Chemical Biology·Joseph C ManimalaJeffrey C Gildersleeve
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Oct 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·A J StreetsA J Leathem

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