Expression of amiloride-blockable sodium channels in Xenopus oocytes.

The American Journal of Physiology
C F Hinton, D C Eaton

Abstract

This report describes the expression of a sodium-selective, amiloride-blockable conductance in Xenopus oocytes that have been injected with RNA prepared from a distal nephron cell line (A6). After injecting the RNA into mature oocytes (stage V or VI) and incubating the oocytes for 2-4 days, the oocytes were examined for amiloride-blockable current. The RNA induced a substantial amiloride-blockable current. Uninjected or water-injected oocytes had no measurable amiloride-blockable current. RNA prepared from aldosterone-treated A6 cells was much more effective in inducing amiloride-blockable sodium current than RNA prepared from aldosterone-depleted A6 cells. Oocytes injected with RNA prepared from mineralocorticoid-depleted cells appeared very similar to water-injected oocytes. The amiloride-blockable current in oocytes has a reversal potential of approximately +50 - +60 mV, which varies 61 mV/decade change in external sodium concentration, suggesting that the current is highly selective for sodium over other ions. In addition, the concentration of amiloride that produces half block of the current is 48 +/- 8 nM. Thus the current expressed in oocytes appears very similar to sodium-selective currents observed from the apical memb...Continue Reading

References

Jan 1, 1975·The Journal of Membrane Biology·D C EatonA M Brown
Jan 1, 1985·Pflügers Archiv : European journal of physiology·S I HelmanL M Baxendale
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L G Palmer, G Frindt
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D J BenosS Sariban-Sohraby
Jul 1, 1972·The Journal of Physiology·D C Eaton
Jul 1, 1980·Physiological Reviews·A D MacknightA Leaf
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P DierksC Weissmann
Jan 1, 1982·The Journal of Membrane Biology·L G PalmerI S Edelman

❮ Previous
Next ❯

Citations

Jun 21, 2014·CNS Neuroscience & Therapeutics·Hillary Quansah, Prosper N'Gouemo
Oct 17, 2002·Journal of Applied Physiology·Sadis MatalonDouglas C Eaton
Aug 1, 1991·Pflügers Archiv : European journal of physiology·B KrollE Frömter
Nov 26, 2008·Journal of Neural Transmission·Jarogniew J LuszczkiStanislaw J Czuczwar
Feb 9, 2000·Pharmacology & Therapeutics·M K Agarwal, M Mirshahi
Jan 1, 1992·Pharmacology & Therapeutics·J P Johnson
Oct 1, 1995·Kidney International·D C EatonB N Ling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.