Expression of aminopeptidase A, an angiotensinase, in glomerular mesangial cells

Hypertension
M TroyanovskayaD P Healy

Abstract

Glomerular mesangial cells are known to express angiotensin II type 1 receptors and contract in response to circulating and/or locally produced angiotensin II. In addition, stimulation of mesangial cell matrix protein synthesis by elevated levels of angiotensin II is known to contribute to the development of glomerulosclerosis. Previously, we reported that mesangial cells were positively immunostained with antiserum directed against aminopeptidase A, the principal angiotensinase in the metabolism of angiotensin II. Here we demonstrate directly that aminopeptidase A is expressed in mesangial cells cultured from rat kidney. First, cultured mesangial cells had measurable aminopeptidase A enzymatic activity. Second, immunoblots for aminopeptidase A were positive for isolated glomeruli and mesangial cells, although two bands were seen for mesangial cells (approximately 138 and 144 kD), and only the larger band was seen for isolated glomeruli and kidney. Third, Northern blot hybridizations of total RNA from mesangial cells or kidney were positive and labeled similarly sized bands. Fourth, reverse transcription-polymerase chain reaction amplification of mesangial cell total RNA yielded a partial cDNA of the expected size that was conf...Continue Reading

References

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Citations

Dec 10, 2008·Nature Clinical Practice. Nephrology·Juan Carlos Q Velez
Sep 1, 1999·Kidney International·A Hus-CitharelC Llorens-Cortes
Feb 14, 2002·Kidney International·Robert F ChouinardKatherine R Tuttle
Dec 2, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Francesca Eleonora BertinettoUNKNOWN European IgA Nephropathy Consortium
Mar 11, 1998·Clinical and Experimental Pharmacology & Physiology·W G HaynesA L Mark
Jan 4, 1998·Kidney International·R Ardaillou, D Chansel
Oct 1, 2015·Journal of Medicinal Chemistry·Lucienne Juillerat-JeanneretJohannes D Aebi
Feb 11, 2014·Orvosi hetilap·Csaba András Dézsi
Feb 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Q JiangD P Healy
Feb 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M TroyanovskayaD P Healy
Oct 1, 1998·The American Journal of Physiology·D ChanselR Ardaillou

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