PMID: 9190209Feb 1, 1997Paper

Expression of an activated rasD gene changes cell fate decisions during Dictyostelium development

Molecular Biology of the Cell
S A LouisG Weeks

Abstract

It has been previously demonstrated that the expression of an activated rasD gene in wild-type Dictyostelium cells results in formation of aggregates with multitips, instead of the normal single tips, and a block in further development. In an attempt to better understand the role of activated RasD development, we examined cell-type-specific gene expression in a strain stably expressing high levels of RasD[G12T]. We found that the expression of prestalk cell-specific genes ecmA and tagB was markedly enhanced, whereas the expression of the prespore cell-specific gene cotC was reduced to very low levels. When the fate of cells in the multitipped aggregate was monitored with an ecmA/lacZ fusion, it appeared that most of the cells eventually adopted prestalk gene expression characteristics. When mixtures of the [G12T]rasD cells and Ax3 cells were induced to differentiate, chimeric pseudoplasmodia were not formed. Thus, although the [G12T]rasD transformant had a marked propensity to form prestalk cells, it could not supply the prestalk cell population when mixed with wild-type cells. Both stalk and spore cell formation occurred in low cell density monolayers of the [G12T]rasD strain, suggesting that at least part of the inhibition of...Continue Reading

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Citations

Jul 23, 2003·Cellular Signalling·Gerald Weeks, George B Spiegelman
Mar 9, 2000·Seminars in Cell & Developmental Biology·S Mohanty, R A Firtel
Jul 14, 1999·Developmental Genetics·J T StegeW F Loomis
Mar 21, 2001·Biochimica Et Biophysica Acta·J R Chubb, R H Insall

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