PMID: 11607423Sep 1, 1993Paper

Expression of an antisense prosystemin gene in tomato plants reduces resistance toward Manduca sexta larvae

Proceedings of the National Academy of Sciences of the United States of America
M L Orozco-CárdenasC A Ryan

Abstract

The growth rates of Manduca sexta (tobacco hornworm) larvae feeding on tomato plants constitutively expressing a prosystemin antisense gene were approximately 3 times higher than growth rates of larvae feeding on nontransformed control plants. The levels of proteinase inhibitor I and inhibitor II proteins in leaves of tomato plants expressing the antisense prosystemin gene remained at undetectable levels until the sixth day of larval feeding and then increased throughout the plants to 100-125 microg/g of leaf tissue after 14 days. In control plants, levels of proteinase inhibitor I and II proteins increased rapidly from the second day of larval feeding and by the eighth day contained levels of 225 microg/g of leaf tissue and 275 microg/g of leaf tissue, respectively, and then increased slowly thereafter. Prosystemin mRNA levels in antisense and control plants after 6 days and 12 days of larval feeding correlated with levels of inhibitor I and II protein levels. These experiments demonstrate that resistance of plants toward an insect pest can be modulated by genetically engineering a gene encoding a component of the inducible systemic signaling system regulating a plant defensive response.

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Citations

Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Schaller, C A Ryan
Dec 17, 2008·Plant Molecular Biology·Rajendra Bari, Jonathan D G Jones
Nov 11, 2003·Trends in Plant Science·Johannes Stratmann
May 29, 2003·Journal of Insect Physiology·C Bolter, M A. Jongsma
Jun 24, 2003·Trends in Plant Science·Johannes W Stratmann
Sep 12, 2002·Annual Review of Plant Biology·André Kessler, Ian T Baldwin
May 4, 2006·Annual Review of Plant Biology·Yoshikatsu Matsubayashi, Youji Sakagami
Jan 1, 1997·Annual Review of Phytopathology·L SticherJ P Métraux
Aug 18, 2004·PLoS Biology·Anke SteppuhnIan T Baldwin
Sep 10, 2013·Journal of Experimental Botany·Markus Albert
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M McConnJ Browse
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E DombrowskiC A Ryan
Jul 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Pramod Kaitheri KandothJohannes W Stratmann
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J RoyoJ J Sánchez-Serrano
Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Clarence A Ryan, Gregory Pearce
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J W Stratmann, C A Ryan
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Ramcharan BhattacharyaClarence A Ryan
Jun 4, 2011·Current Opinion in Plant Biology·Yube Yamaguchi, Alisa Huffaker
Nov 4, 2006·Plant, Cell & Environment·Carla Sánchez-HernándezJohn P Délano-Frier
May 21, 2005·Biochimica Et Biophysica Acta·Gen-ichiro ArimuraWilhelm Boland
Oct 4, 2014·Frontiers in Plant Science·Daniel V SavatinFelice Cervone
Mar 1, 2016·Plant, Cell & Environment·Yu-Chi LiShih-Tong Jeng
Aug 15, 2019·Plant Signaling & Behavior·Renata Ranielly Pedroza CruzRailene Herica Carlos Rocha Araújo
Nov 24, 2017·Protein Science : a Publication of the Protein Society·Martina BuonannoSimona Maria Monti
Feb 21, 2019·Annual Review of Plant Biology·Matthias Erb, Philippe Reymond
Apr 1, 2020·F1000Research·Chun-Yu Chen, Ying-Bo Mao
Jan 29, 2020·Journal of Chemical Ecology·Laquita GrissettJohannes W Stratmann

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