PMID: 9177195Jun 10, 1997Paper

Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities

Proceedings of the National Academy of Sciences of the United States of America
F ChaumontM J Chrispeels

Abstract

The rd28 gene of Arabidopsis thaliana encodes a water channel protein, or aquaporin, of the plasma membrane. A construct in which transcription of the rd28 cDNA is controlled by the Dictyostelium actin15 promoter was transformed into Dictyostelium discoideum cells. Transformants contained RD28 protein in their plasma membranes. When shifted to a low-osmotic-strength buffer, cells expressing rd28 swelled rapidly and burst, indicating that the plant aquaporin allowed rapid water entry in the amoebae. The rate of osmotic lysis was a function of the osmotic pressure of the buffer. We also selected transformants in which the expression of the rd28 cDNA is driven by the promoter of the prespore cotB gene. These transformants accumulated rd28 mRNA uniquely in prespore cells. In low-osmotic-strength buffer, the cotB::rd28 cells aggregated and formed normally proportioned slugs but failed to form normal fruiting bodies. The number of spores was reduced 20-fold, and the stalks of the fruiting bodies were abnormally short. The consequences of expressing RD28 in prespore cells could be partially overcome by increasing the osmolarity of the medium. Under these conditions, the cotB::rd28 cells formed fruiting bodies of more normal appearance...Continue Reading

References

Nov 1, 1968·Journal of Bacteriology·D A Cotter, K B Raper
Aug 1, 1995·Current Opinion in Cell Biology·H M Kwon, J S Handler
Sep 1, 1995·Developmental Biology·R Escalante, W F Loomis
Aug 1, 1994·The Plant Journal : for Cell and Molecular Biology·W KammerloherA R Schäffner
Nov 1, 1993·Developmental Biology·G Shaulsky, W F Loomis
Jan 12, 1996·Science·H KuwayamaP J Van Haastert
May 1, 1996·The Biochemical Journal·N R CohenH F Lodish
Jan 1, 1996·Annual Review of Physiology·L S King, P Agre
Nov 15, 1996·Science·G P MooreP Breeze
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·G ShaulskyW F Loomis
Nov 1, 1989·Plant Physiology·K D JohnsonM J Chrispeels

❮ Previous
Next ❯

Citations

Apr 24, 2009·Molecular and Cellular Biochemistry·Sarah J Annesley, Paul R Fisher
Dec 19, 2003·FEMS Microbiology Letters·Yohko Yamada, Masazumi Sameshima
Jun 13, 2002·Physiologia Plantarum·Isabel BaigesAlberto Mas
Nov 15, 2011·Biochemical and Biophysical Research Communications·Veronika SchönitzerIngrid M Weiss
Feb 15, 2012·Biochemical and Biophysical Research Communications·Stefan KaufmannMotomu Tanaka
Apr 5, 2000·Biochimica Et Biophysica Acta·A J Miller, J J Zhou
Aug 21, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ranjana AryaKulvinder Singh Saini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.