PMID: 2120194Oct 1, 1990Paper

Expression of an aromatic-dependent decarboxylase which provides growth-essential CO2 equivalents for the acetogenic (Wood) pathway of Clostridium thermoaceticum

Journal of Bacteriology
T D HsuH L Drake

Abstract

The acetogen Clostridium thermoaceticum generates growth-essential CO2 equivalents from carboxylated aromatic compounds (e.g., 4-hydroxybenzoate), and these CO2 equivalents are likely integrated into the acetogenic pathway (T. Hsu, S. L. Daniel, M. F. Lux, and H. L. Drake, J. Bacteriol. 172:212-217, 1990). By using 4-hydroxybenzoate as a model substrate, an assay was developed to study the expression and activity of the decarboxylase involved in the activation of aromatic carboxyl groups. The aromatic-dependent decarboxylase was induced by carboxylated aromatic compounds in the early stages of growth and was not repressed by glucose or other acetogenic substrates; nonutilizable carboxylated aromatic compounds did not induce the decarboxylase. The decarboxylase activity displayed saturation kinetics at both whole-cell and cell extract levels, was sensitive to oxidation, and was not affected by exogenous energy sources. However, at the whole-cell level, metabolic inhibitors decreased the decarboxylase activity. Supplemental biotin or avidin did not significantly affect decarboxylation. The aromatic-dependent decarboxylase was specific for benzoates with a hydroxyl group in the para position of the aromatic ring; the meta position...Continue Reading

References

May 1, 1989·Applied and Environmental Microbiology·H C YangH L Drake
Jan 1, 1988·Annual Review of Microbiology·W C Evans, G Fuchs
Jan 1, 1986·Annual Review of Microbiology·L G Ljungdahl
Aug 1, 1987·Applied and Environmental Microbiology·M D SavageH L Drake
Nov 1, 1988·Applied and Environmental Microbiology·S F LajoieM J Wolin
Jan 1, 1986·Journal of Bacteriology·M D Savage, H L Drake
Dec 30, 1983·Biochimica Et Biophysica Acta·T A Krulwich
Aug 1, 1984·Journal of Bacteriology·L L Lundie, H L Drake
Sep 1, 1951·Archives of Biochemistry and Biophysics·E F PHARES
Jun 1, 1985·Applied and Environmental Microbiology·D R MartinH L Drake
Jan 1, 1986·Applied and Environmental Microbiology·A C Frazer, L Y Young
Jun 1, 1942·Journal of Bacteriology·F E FontaineG J Ritter

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Citations

Dec 15, 2004·Critical Reviews in Biochemistry and Molecular Biology·Stephen W Ragsdale
Apr 2, 2008·Annals of the New York Academy of Sciences·Harold L DrakeSteven L Daniel
Jul 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Dan YuBo Yu
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·S W Ragsdale
Mar 22, 2007·FEMS Microbiology Ecology·Kimberly L CookKinchel C Doerner
Dec 22, 2009·Chemical Society Reviews·Silvia M GlueckKurt Faber
Aug 6, 2019·Advanced Synthesis & Catalysis·Stefan E PayerSilvia M Glueck
Jan 1, 1993·Applied and Environmental Microbiology·D P Cunningham, L L Lundie
Dec 1, 1993·Journal of Bacteriology·C SeifritzH L Drake
Sep 1, 1993·Applied and Environmental Microbiology·S L Daniel, H L Drake
Nov 1, 1994·Applied and Environmental Microbiology·X Zhang, J Wiegel
Jul 14, 2004·Research in Microbiology·Harold L Drake, Steven L Daniel
Jan 6, 2005·Research in Microbiology·Harold L Drake, Steven L Daniel

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