Expression of and secretion through the Aeromonas salmonicida type III secretion system

Microbiology
Roger O EbanksNeil W Ross

Abstract

Aeromonas salmonicida subsp. salmonicida is the aetiological agent of furunculosis, a disease of farmed and wild salmonids. The type III secretion system (TTSS) is one of the primary virulence factors in A. salmonicida. Using a combination of differential proteomic analysis and reverse transcriptase (RT)-PCR, it is shown that A. salmonicida A449 induces the expression of TTSS proteins at 28 degrees C, but not at its more natural growth temperature of 17 degrees C. More modest increases in expression occur at 24 degrees C. This temperature-induced up-regulation of the TTSS in A. salmonicida A449 occurs within 30 min of a growth temperature increase from 16 to 28 degrees C. Growth conditions such as low-iron, low pH, low calcium, growth within the peritoneal cavity of salmon and growth to high cell densities do not induce the expression of the TTSS in A. salmonicida A449. The only other known growth condition that induces expression of the TTSS is growth of the bacterium at 16 degrees C in salt concentrations ranging from 0.19 to 0.38 M NaCl. It is also shown that growth at 28 degrees C followed by exposure to low calcium results in the secretion of one of the TTSS effector proteins. This study presents a simple in vitro model fo...Continue Reading

References

Jul 1, 1992·Journal of Bacteriology·P L Haddix, S C Straley
Oct 1, 1989·Molecular Microbiology·G R CornelisJ C Vanooteghem
Oct 1, 1981·Journal of Bacteriology·E E IshiguroT J Trust
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WattiauG R Cornelis
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Aug 26, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J M Janda, S L Abbott
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·G R CornelisI Stainier
Feb 13, 1999·Molecular Microbiology·G FrankelS Knutton
Feb 27, 1999·Current Opinion in Microbiology·G T Nhieu, P J Sansonetti
Apr 1, 2000·Microbes and Infection·M E Konkel, K Tilly
Jul 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M J FiguerasA Martínez-Murcia
Oct 6, 2000·Annual Review of Microbiology·G R Cornelis, F Van Gijsegem
Feb 15, 2002·Journal of Bacteriology·Briana M Young, Glenn M Young
Oct 11, 2002·Journal of Bacteriology·Sarah E BurrJoachim Frey
Sep 13, 2003·Fish & Shellfish Immunology·Vera LundChristian Syvertsen
Nov 1, 1956·Journal of Bacteriology·G E HELZC PANOS
Jan 23, 2004·Diseases of Aquatic Organisms·Sarah E BurrJoachim Frey
Mar 9, 2004·Journal of Clinical Microbiology·M R ChacónM J Figueras
Mar 25, 2004·Infection and Immunity·Marcelo P SirciliVanessa Sperandio
Jun 19, 2004·Journal of Bacteriology·Kimberly A Walker, Virginia L Miller

❮ Previous
Next ❯

Citations

Aug 18, 2009·Applied and Environmental Microbiology·Silvia VilchesSusana Merino
Jan 24, 2007·Journal of Bacteriology·Yael Litvak, Zvi Selinger
Oct 4, 2012·Biochimica Et Biophysica Acta·Elke UribeK Vanya Ewart
Oct 29, 2015·Veterinary Microbiology·Esteban SotoJohn D Hansen
Jul 9, 2013·Microbiology·Stéphanie Dallaire-DufresneSteve J Charette
Jun 5, 2013·Mobile Genetic Elements·Katherine H TanakaSteve J Charette
Aug 21, 2013·Mobile Genetic Elements·Mélanie V TrudelSteve J Charette
Jan 7, 2014·Journal of Microbiological Methods·Stéphanie Dallaire-DufresneSteve J Charette
Sep 10, 2016·Frontiers in Microbiology·Cody R Rasmussen-IveyMark R Liles
Jun 5, 2019·PloS One·Luiz Thiberio RangelJohann Peter Gogarten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.