Expression of annexin II and stromal tenascin C promotes epithelial to mesenchymal transition and correlates with distant metastasis in pancreatic cancer

International Journal of Molecular Medicine
Naoko YoneuraMasayuki Ohtsuka

Abstract

The interaction between cancer cells and stromal components contributes to cancer invasion and metastasis in pancreatic ductal adenocarcinoma (PDAC). The present study investigated the role of the correlation between annexin II (ANX2) and stromal tenascin C (TNC) with the progression of PDAC. The functions of the expression ANX2 and TNC were assessed in in vitro experiments using mouse and human PDAC cells, and the clinical effect was analyzed using immunohistochemistry with surgically resected PDAC tissues. The effects on epithelial to mesenchymal transition (EMT), invasion, putative cancer stemness, and anoikis resistance were examined in vitro using murine precancerous pancreatic intraepithelial neoplasia (PanIN) cells and murine and human invasive PDAC cells with ANX2 knockdown using specific small interfering RNA (siRNA)s and recombinant TNC (rTNC). ANX2 was expressed at a high level in primary PanIN cells and invasive PDAC cells, compared with the levels in liver metastatic PDAC cells. In the ANX2‑knockdown cells, there were fewer cells with a morphological mesenchymal appearance in three‑dimensional culture and invasion was reduced compared with that in the control cells. Morphological change into the mesenchymal phenoty...Continue Reading

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Methods Mentioned

BETA
surgical resection
Protein
electrophoresis
transfection

Software Mentioned

AxioVision
JMP PRO
ImageJ

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