Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens

Biotechnology and Applied Biochemistry
Parthasarathy BalajiMookambeswaran A Vijayalakshmi

Abstract

Therapeutic antibodies against tumor necrosis factor alpha (TNFα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, Crohn's diseases, and so on. But associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. Single-chain variable fragment (scFv) can negate the limitations tagged with the anti-TNFα therapeutics to a greater extent. In the present study, Spirodela punctata plants were transformed with anti-TNFα through in planta transformation using Agrobacterium tumefaciens strain, EHA105. Instead of cefotaxime, garlic extract (1 mg/mL) was used to remove the agrobacterial cells after cocultivation. To the best of our knowledge, this report shows for the first time the application of plant extracts in transgenic plant development. 95% of the plants survived screening under hygromycin. ScFv cDNA integration in the plant genomic DNA was confirmed at the molecular level by PCR. The transgenic protein expression was followed up to 10 months. Expression of scFv was confirmed by immunodot blot. Protein expression levels of up to 6.3% of total soluble protein were observed. β-Glucuronidase and...Continue Reading

References

Mar 25, 1991·Nucleic Acids Research·K EdwardsC Thompson
May 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Sulkowski
Oct 21, 1988·Science·R E BirdM Whitlow
Jul 24, 1987·Nucleic Acids Research·R TöpferH H Steinbiss
Sep 1, 1994·Plant Molecular Biology·P HajdukiewiczP Maliga
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
Mar 9, 2004·Current Opinion in Plant Biology·Rainer FischerRichard M Twyman
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniela Todorova-BalvayMookambeswaran Vijayalakshmi
Oct 12, 2005·Biotechnology Annual Review·Anne-Marie Stomp
Nov 28, 2006·Nature Biotechnology·Kevin M CoxLynn F Dickey
May 3, 2007·The Biochemical Journal·Mengyuan LiuHualiang Huang
May 12, 2007·Plant Cell Reports·Ron VunshGeorges Freyssinet
Dec 1, 2007·Biotechnology and Applied Biochemistry·Mengyuan LiuHualiang Huang
Apr 5, 2008·Protein Engineering, Design & Selection : PEDS·Lucy J HoltIan M Tomlinson
Apr 27, 2010·Journal of Chromatography. a·Rajasekar R Prasanna, Mookambeswaran A Vijayalakshmi
Jan 1, 2011·Horticulture, Environment and Biotechnology·Suk-Min KoSuk Weon Kim

❮ Previous
Next ❯

Citations

Feb 10, 2020·Molecular Biotechnology·Padikara Kutty Satheeshkumar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.