PMID: 6410152Jan 1, 1983Paper

Expression of antibiotic resistance genes from Escherichia coli in Bacillus subtilis

Molecular & General Genetics : MGG
J KreftW Goebel

Abstract

Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferase (cat) gene from the E. coli plasmids were not transcribed and therefore not expressed in B. subtilis. However, tetracycline resistance from the E. coli plasmids was expressed in B. subtilis. Transcription of the tetracycline resistance gene(s) started in B. subtilis at or near the original E. coli promoter, the sequence of which is almost identical with the sequence recognized by sigma 55 of B. subtilis RNA polymerase.

References

Jun 1, 1978·Molecular & General Genetics : MGG·J KreftW Goebel
Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 29, 1977·Molecular & General Genetics : MGG·H MayerW Goebel
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·J G Sutcliffe
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S D Ehrlich
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T J Gryczan, D Dubnau
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S D Ehrlich
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S N CohenR B Helling
Jun 4, 1974·Biochemistry·V GlisinC Byus
Jun 11, 1981·Nucleic Acids Research·D R Russell, G N Bennett
Oct 25, 1981·Journal of Molecular Biology·G Lee, J Pero
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D S GoldfarbR H Doi
Sep 1, 1981·Cell·R Losick, J Pero
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Horinouchi, B Weisblum
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D Stüber, H Bujard

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·G EitnerJ Hofemeister
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D G GuineyC E Davis
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
Feb 12, 2015·FEMS Microbiology Reviews·Nienke Buddelmeijer
Jan 1, 1986·Journal of Basic Microbiology·G Eitner-Mönke, R Manteuffel
Jul 1, 1986·Plasmid·G GrandiS Toma

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