PMID: 6170982Sep 1, 1981Paper

Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
A R DavisD G Kleid

Abstract

Antigenic determinants of influenza virus hemagglutinin were expressed in Escherichia coli. DNA coding for presequences of hemagglutinin were removed and an ATG codon was placed before DNA coding for mature hemagglutinin. A number of expression plasmids were constructed in which various segments of this reconstructed hemagglutinin DNA were fused to DNA coding for bacterial beta-galactosidase. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.

References

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Citations

Jan 1, 1989·Plant Molecular Biology·P D BeremandD J Guerra
Feb 1, 1985·Virus Research·K LundströmR F Pettersson
Jan 1, 1983·Biotechnology Advances·J D Friesen, G An
Oct 4, 1984·The New England Journal of Medicine·N C Engleberg, B I Eisenstein
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J R HartmanG C Fareed
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A R DavisD P Nayak
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J F YoungM Rosenberg
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A JabbarD P Nayak
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A YoungR W Davis
Feb 16, 1987·European Journal of Biochemistry·K P NambiarS A Benner
Jul 28, 2010·PloS One·José M Aguilar-YáñezMario M Alvarez
Jul 29, 1983·Journal of Immunological Methods·R ArnonC O Jacob
Mar 11, 1982·Nature·R G WebsterG C Schild
May 1, 1989·Advances in Dental Research·M A TaubmanJ D Hillman
Jun 12, 2013·Biotechnology Progress·Pamela B Sánchez-ArreolaMario M Alvarez
Apr 3, 2020·ACS Applied Materials & Interfaces·Jhanvi SharmaTrevor Douglas

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