Expression of antimicrobial peptides under control of a camalexin-biosynthetic promoter confers enhanced resistance against Pseudomonas syringae

Phytochemistry
Alexandra ChapmanErich Glawischnig

Abstract

In Arabidopsis thaliana phytoalexin biosynthesis is tightly regulated. The camalexin biosynthetic gene CYP71B15/PAD3 is highly expressed in response to pathogens and specific abiotic triggers, while constitutive expression is very low. Based on this property we expressed artificial antimicrobial peptides under control of the CYP71B15 promoter avoiding potential toxic effects to the plant related to constitutive expression. Significant and substantial growth inhibition of Pseudomonas syringae was observed, demonstrating that expression of these peptides under control of a phytoalexin promoter is an effective approach for enhancement of resistance against bacterial pathogens.

References

Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Glazebrook, F M Ausubel
May 20, 1999·Biopolymers·F García-OlmedoP Rodríguez-Palenzuéla
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Jan 25, 2002·Nature·Michael Zasloff
Nov 26, 2002·Planta·Bart P H J ThommaKarin Thevissen
Aug 22, 2003·Journal of Internal Medicine·H G Boman
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Mar 25, 2006·Proceedings. Biological Sciences·Gabriel G PerronGraham Bell
Jun 13, 2006·Plant Physiology·Regina SchuheggerErich Glawischnig
Jun 14, 2006·Journal of Plant Physiology·Regina SchuheggerErich Glawischnig
Jun 17, 2006·Nature Reviews. Microbiology·Andreas Peschel, Hans-Georg Sahl
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Mar 21, 2007·FEMS Microbiology Letters·Emilio Montesinos
Apr 29, 2008·Annual Review of Phytopathology·Jose F MarcosBelén López-García
Oct 30, 2008·Nucleic Acids Research·Guangshun WangZhe Wang
Jun 16, 2009·Phytochemistry·Thomas Rauhut, Erich Glawischnig
Oct 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Angelika MustrophJulia Bailey-Serres
Feb 16, 2011·Research in Microbiology·Gergely MarótiPeter Mergaert
Mar 16, 2011·Biochemistry Research International·Patrícia Barbosa PelegriniMaria Fátima Grossi-de-Sa
May 28, 2011·Natural Product Reports·Miriam WilmesKarin Thevissen
Sep 7, 2011·Journal of Molecular Microbiology and Biotechnology·Sylvia A Baltzer, Melissa H Brown
Dec 17, 2011·Nature Reviews. Drug Discovery·Christopher D FjellGisbert Schneider
Oct 20, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min-Duk SeoBong-Jin Lee
Dec 18, 2012·Plant Molecular Biology·Benjamin ZeitlerChristian Lindermayr
Aug 21, 2013·PloS One·Benjamin ZeitlerChristian Lindermayr
Apr 30, 2014·Current Opinion in Solid State & Materials Science·Nathan W Schmidt, Gerard C L Wong
Nov 8, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Philippe JeandetJérôme Crouzet
Jan 15, 2015·Applied Microbiology and Biotechnology·João Pinto da CostaRui Vitorino
Jan 30, 2015·Canadian Journal of Microbiology·Chandra Datta SumiYoung Tae Hahm
May 29, 2015·Journal of Animal Science and Biotechnology·Hao XiaoYulong Yin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved