Expression of antisense RNA fails to inhibit influenza virus replication.

Virus Research
J M LeiterP Palese

Abstract

Cell lines were constructed which permanently express influenza virus-specific RNA. Two approaches were followed. C127 cells were transformed with bovine papilloma virus (BPV) vectors and the resulting cell lines were found to inhibit the replication of influenza virus at low multiplicity of infection (MOI 0.05). However, examination of cellular RNA using single-stranded probes revealed the presence of both (+)sense and antisense RNA transcripts (45-70 copies per cell). In this BPV-based system the inhibitory activity appeared to be associated with a non-specific, interferon (IFN)-mediated effect. In the second approach, an expression system was used which involved 293 cells, a chimeric human cytomegalovirus (CMV)/human immunodeficiency virus (HIV) promoter, and methotrexate- (Mtx)-mediated gene amplification. Cells were found to express up to 7500 copies of influenza virus-specific RNA per cell at a steady state level. In this system no RNA transcripts of the opposite orientation were found. However, all cell lines permanently expressing either (-)sense or (+)sense viral RNA failed to reduce influenza virus titers in a multi-cycle replication experiment (MOI 0.01).

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P C Zamecnik, M L Stephenson
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A HirashimaM Inouye
May 1, 1988·Genes & Development·J Walder
Feb 1, 1989·Virus Research·R A LiM Krystal
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Aruffo, B Seed
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M KrystalP Palese
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C C SmithP O Ts'o
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LemaitreB Lebleu
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J GoodchildP C Zamecnik
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalP C Zamecnik
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M MatsukuraS Broder
Jun 4, 1974·Biochemistry·V GlisinC Byus
Jan 1, 1982·Annual Review of Biochemistry·P Lengyel
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P DavanlooF W Studier

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Citations

May 20, 1999·Antiviral Research·V N LazarevB S Naroditsky
Jun 28, 2000·Nucleic Acids Research·A CarneroD H Beach
Dec 20, 2002·Nucleic Acids Research·Eugene Y KohGeorge Q Daley
May 15, 2002·Microbiology and Immunology·Yasushi OhtsuTetsuya Toyoda
Oct 30, 2012·Analytical Biochemistry·Qiang WangTetsuya Toyoda
Jan 1, 1995·Antisense Research and Development·H A ThieringerM Inouye

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