PMID: 9163609May 1, 1997Paper

Expression of Apo-1/Fas (CD95), Bcl-2, Bax and Bcl-x in myeloma cell lines: relationship between responsiveness to anti-Fas mab and p53 functional status

British Journal of Haematology
A EgleR Greil

Abstract

The down-regulation of apoptosis may be an essential mechanism for tumour cell expansion in slowly proliferating tumours such as multiple myeloma. We studied eight myeloma cell lines for the presence of Bcl-2, which inhibits apoptosis, of Bax, which counteracts Bcl-2, of Bcl-x(L) and Bcl-x(S), which act in an anti- and pro-apoptotic fashion, respectively, and of Apo-1/Fas, which induces programmed cell death, when activated by the Apo-1/Fas ligand or the relevant monoclonal antibody (mab). All cell lines constitutively expressed homogenous amounts of Bcl-2, but displayed different amounts of Bax and Bcl-x proteins. The Apo-1/Fas antigen could be detected in seven out of eight myeloma lines, but expression levels varied considerably. The relative expression levels of Apo-1/Fas correlated with that of Bax, but not with that of Bcl-2 or Bcl-x subtypes. Furthermore, the effectiveness of the Apo-1/Fas mab was associated with the relative expression levels of the Apo-1/Fas and with that of the Bax antigen, but not with that of the Bcl-2 and Bcl-x antigens. We further showed that wild-type p53 function is not required for Apo-1/Fas-induced apoptosis, nor is it necessary for the expression of Bax or Apo-1/Fas antigens in myeloma. In co...Continue Reading

Citations

Nov 25, 1998·British Journal of Haematology·T OtsukiA Ueki
Dec 8, 1998·The Journal of Experimental Medicine·M MüllerP H Krammer
Oct 2, 2009·Journal of Clinical Biochemistry and Nutrition·Quanxin WangSumio Watanabe
Sep 5, 2003·International Journal of Hematology·Franco SilvestrisFranco Dammacco
Jul 3, 2008·Cancer Immunology, Immunotherapy : CII·Jun WeiAmy B Heimberger
Mar 6, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Steven K Lundy
Feb 3, 2009·British Journal of Haematology·Michel JourdanBernard Klein
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·I V FilippovichJ F Chatal
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C R ScogginsS D Leach
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