Expression of apolipoprotein B in the kidney attenuates renal lipid accumulation.

The Journal of Biological Chemistry
Marcin KrzystanekLars B Nielsen

Abstract

The ability to produce apolipoprotein (apo) B-containing lipoproteins enables hepatocytes, enterocytes, and cardiomyocytes to export triglycerides. In this study, we examined secretion of apoB-containing lipoproteins from mouse kidney and its putative impact on triglyceride accumulation in the tubular epithelium. Mouse kidney expressed both the apoB and microsomal triglyceride transfer protein genes, which permit lipoprotein formation. To examine de novo lipoprotein secretion, kidneys from human apoB-transgenic mice were minced and placed in medium with (35)S-amino acids. Upon sucrose gradient ultracentrifugation of the labeled medium, fractions were analyzed by apoB immunoprecipitation. (35)S-Labeled apoB100 was recovered in approximately 1.03-1.04 g/ml lipoproteins (i.e. similar to the density of plasma low density lipoproteins). Immunohistochemistry of kidney sections suggested that apoB mainly is produced by tubular epithelial cells. ApoB expression in the kidney cortex was reduced approximately 90% in vivo by treating wild type mice with apoB-antisense locked nucleic acid oligonucleotide. Inhibition of apoB expression increased fasting-induced triglyceride accumulation in the kidney cortex by 20-25% (p = 0.008). Cholestero...Continue Reading

References

Nov 1, 1990·Circulation·S G Young
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L A DemmerJ I Gordon
Feb 28, 1986·Biochimica Et Biophysica Acta·J R Wetterau, D B Zilversmit
Apr 1, 1994·Current Opinion in Lipidology·R E Gregg, J R Wetterau
Dec 1, 1993·The Journal of Clinical Investigation·M F LintonS G Young
Mar 19, 1993·Science·L M ObeidY A Hannun
Mar 7, 1998·The Journal of Biological Chemistry·M ShimabukuroR H Unger
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M ShimabukuroR H Unger
Apr 29, 1998·The Journal of Clinical Investigation·J BorénS G Young
Mar 4, 1999·The Journal of Nutrition·R L WalzemR L Hamilton
Apr 15, 1999·Journal of the American Society of Nephrology : JASN·E I ChristensenS K Moestrup
Jan 31, 2002·Annual Review of Medicine·Roger H Unger
Sep 18, 2002·Kidney International·Didier PortillaShenyang Li
Jul 17, 2003·Endocrinology·Christina ChristoffersenLars B Nielsen
Nov 13, 2003·American Journal of Physiology. Renal Physiology·Shenyang LiDidier Portilla
Oct 27, 2004·The Journal of Biological Chemistry·Eva M MadsenLars B Nielsen
Dec 22, 2004·Kidney International·Richard A ZagerSherry Y Hanson
Mar 3, 2006·The Journal of Clinical Investigation·Liliane Michalik, Walter Wahli
Mar 4, 2006·Kidney International·H Birn, E I Christensen
May 5, 2006·Kidney International·D PortillaR D Beger
Mar 9, 2007·The Proceedings of the Nutrition Society·Patrick Schrauwen
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Christian A BangLars B Nielsen
May 27, 2008·Metabolism: Clinical and Experimental·Marie L S Lindegaard, Lars B Nielsen
May 20, 2009·Experimental Eye Research·Masashi FujiharaJames T Handa

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Citations

May 22, 2010·Current Opinion in Nephrology and Hypertension·Ion Alexandru Bobulescu
Jul 20, 2012·Physiological Reviews·Nada A Abumrad, Nicholas O Davidson
Feb 23, 2012·Nutrition & Metabolism·M Mahmood HussainJahangir Iqbal
Jun 17, 2014·Journal of Reproductive Immunology·Aafke P A van MontfoortUwe J F Tietge
Mar 29, 2014·Scandinavian Journal of Clinical and Laboratory Investigation·Emil D BartelsLars B Nielsen
Apr 17, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Phillip M MarvynRobin E Duncan
Oct 4, 2016·Critical Reviews in Clinical Laboratory Sciences·Meghan T Walsh, M Mahmood Hussain
Apr 14, 2017·Journal of Lipid Research·Diego ScerboIra J Goldberg
Mar 4, 2017·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Xin LiuGeorge Liu
Nov 13, 2014·Journal of Lipid Research·Emile Levy
Apr 21, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Richard LehnerAriel D Quiroga
Sep 12, 2015·Circulation Research·Emil D BartelsLars B Nielsen
Aug 5, 2018·Journal of Lipid Research·Nicholas N LyssenkoVenkata Ramana Murthy Chavali
May 8, 2020·Molecular Nutrition & Food Research·Danijela Vojnović MilutinovićNataša Veličković

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