Expression of apoptosis-related genes in the organ of Corti, modiolus and stria vascularis of newborn rats

Brain Research
Johann GrossBirgit Mazurek

Abstract

Cell death in the inner ear tissues is an important mechanism leading to hearing impairment. Here, using microarrays and real-time RT-PCR we analyzed expression of selected apoptosis-related genes in rat's inner ear. We determined the gene expression in tissues freshly isolated from neonatal rats (3-5 days old) and compared it to that of explants cultured for 24 h under normoxic or hypoxic conditions. For the analyses, we used pooled samples of the organ of Corti (OC), modiolus (MOD) and stria vascularis (SV), respectively. We observed region-specific changes in gene expression between the fresh tissues and the normoxic culture. In the OC, expression of the proapoptotic genes caspase-2, caspase-3, caspase-6 and calpain-1 was downregulated. In the MOD, the antioxidative defense SOD-2 and SOD-3 were upregulated. In the SV, caspase-2, caspase-6, calpain-1 and SOD-3 were downregulated and SOD-2 upregulated. We speculate that these changes could reflect survival shift in transcriptome of inner ear explants tissues under in vitro conditions. With the exception of SOD-2, hypoxic culture conditions induced the same changes in gene expression as the normoxic conditions indicating that culture preparation is likely the dominating factor,...Continue Reading

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Citations

May 27, 2011·Cellular and Molecular Neurobiology·Johann GrossBirgit Mazurek
Apr 12, 2011·Human Molecular Genetics·Nadine KosslerMateusz Kolanczyk
Feb 19, 2009·Journal of Negative Results in Biomedicine·Birgit MazurekPhilipp P Caffier
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Jul 14, 2011·Neuromolecular Medicine·D EkuniM Morita
Mar 31, 2011·Hearing Research·Birgit MazurekJohann Gross
Mar 15, 2011·Molecular and Cellular Neurosciences·Alina SmorodchenkoElena E Pohl
Nov 12, 2010·Cell Biology International·Li YuHsiao Chang Chan
Mar 12, 2010·Journal of Clinical Pharmacology·Kenneth MaieseJinling Hou
Jun 6, 2018·Frontiers in Neuroscience·Moritz GröschelAgnieszka J Szczepek

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