Expression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose in vitro.

Journal of the American Society of Nephrology : JASN
Kar Neng LaiJoseph C K Leung

Abstract

Aquaporin (AQP) is a family of water channels that are highly selective for the passage of water and occasionally glycerol. In previous studies, only AQP1 was found in human peritoneal endothelial cells in both control subjects and patients on peritoneal dialysis. As human peritoneal mesothelial cells (HPMC) play an important role in dialysis adequacy and fluid balance in continuous ambulatory peritoneal dialysis patients, this study examined whether AQP1 is present in HPMC. It was found that AQP1 mRNA and protein are present in HPMC constitutively. The localization of AQP1 protein in peritoneal mesothelial cells was confirmed by double immunohistochemical staining of the mesothelial lining of human peritoneal membrane. More important, the expression of AQP1 in HPMC is not constitutive and the transcription and biosynthesis of AQP1 in HPMC is inducible by osmotic agents such as glucose and mannitol. There was significant enhancement of AQP1 biosynthesis upon exposure to glucose in a time- and dose-dependent manner (P < 0.0001). Similar findings were observed in the AQP1 biosynthesis by an endothelial cell line, EA.hy 926. Of particular interest, the upregulation in AQP1 mRNA or biosynthesis in mesothelial cells was always signi...Continue Reading

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Citations

Feb 5, 2013·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Jacek WaniewskiBengt Lindholm
Sep 17, 2002·Kidney International. Supplement·Laurinda A CookerCatherine M Hoff
Mar 14, 2003·The Journal of Pathology·Kar Neng LaiHui Yao Lan
Apr 10, 2012·The International Journal of Biochemistry & Cell Biology·Ai Ing LimJoseph C K Leung
Feb 12, 2004·Kidney International. Supplement·Catherine M Hoff
Aug 11, 2004·The Veterinary Journal·Ali MobasheriDavid Marples
Jan 30, 2004·The Journal of Laboratory and Clinical Medicine·Lazaro GotloibAvshalom Shostak
Jun 24, 2004·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Gerrit SchoenickeJoerg Plum
Jul 18, 2006·Biochimica Et Biophysica Acta·Cécile Héliès-ToussaintAlain Grynberg
Sep 14, 2007·Kidney International·J NieX Q Yu
Sep 28, 2007·Neoplasia : an International Journal for Oncology Research·Yasuhiko HayashiMarsha J Merrill
Dec 15, 2007·Pflügers Archiv : European journal of physiology·Tomoya Nishino, Olivier Devuyst
Jul 3, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ana Marta GomesTeresa García Falcón

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