Abstract
This study was designed to examine whether aquaporin 4 (AQP4) is present in the chicken oviduct, and if so, whether its expression changes during pause in laying induced by tamoxifen (TMX; oestrogen receptor modulator) treatment. The control chickens were injected with a vehicle (ethanol) and the experimental ones with TMX at a dose of 6 mg/kg of body weight. Birds were treated daily until complete cessation of egg laying. The oviductal parts, that is the infundibulum, magnum, isthmus, shell gland and vagina were isolated from hens on day 8 of the experiment, and subsequently, the gene and protein expressions of AQP4 in tissues were examined by real-time PCR and Western blot, respectively. Immunohistochemical localization of AQP4 in the wall of the chicken oviduct was also investigated. Both mRNA and protein of AQP4 were found in all segments of the chicken oviduct. The relative expression [RQ] of AQP4 was the highest in the infundibulum and the vagina and the lowest, less detectable, in the magnum and isthmus. The pattern of AQP4 protein expression was similar to that of mRNA. Treatment of hens with TMX decreased the mRNA and protein levels of AQP4 in the oviduct. Immunohistochemistry demonstrated tissue and cell-dependent loc...Continue Reading
References
May 1, 1991·Poultry Science·Y NysA D Mills
Apr 5, 2001·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·B J GannonC J Verco
Jul 12, 2003·Biology of Reproduction·Elizabeth M JablonskiYvette M Huet-Hudson
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mahmood Amiry-MoghaddamOle P Ottersen
Oct 6, 2005·Trends in Endocrinology and Metabolism : TEM·Dawne C Dougherty, Michel M Sanders
Jul 15, 2006·Human Reproduction Update·He-Feng HuangYing-Ying Ma
Sep 27, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mika KobayashiTaisen Iguchi
Dec 23, 2006·Placenta·M De FalcoA De Luca
Mar 3, 2007·Journal of Anatomy·Jinxiong LiuQiusheng Chen
Jan 25, 2008·The Biochemical Journal·Udo MoehrenFrank Claessens
Jul 16, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K SugiuraN Saito
Jun 24, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mariusz T SkowronskiSoren Nielsen
Oct 21, 2009·Proteins·Yi Wang, Emad Tajkhorshid
Sep 11, 2010·Reproductive Biology and Endocrinology : RB&E·Mariusz T Skowronski
Feb 25, 2011·Cell and Tissue Research·Keiji YoshimuraNoboru Saito
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mariusz T SkowronskiSoren Nielsen
Jul 1, 2011·The Journal of Clinical Endocrinology and Metabolism·Li-Bo ZouHe-Feng Huang
Mar 23, 2013·PloS One·Davide BascoAntonio Frigeri
Sep 27, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hiroko Nishimura, Yimu Yang
Oct 26, 2014·Journal of Ovarian Research·Anupama TiwariRamesh Ramachandran
Nov 2, 2014·Journal of Neuroinflammation·Heling ChuQiang Dong
Jan 2, 2015·Frontiers in Bioscience (Landmark Edition)·Cui ZhuGuoyao Wu
Jan 26, 2016·Domestic Animal Endocrinology·C YangG Song
Mar 30, 2016·Reproduction in Domestic Animals = Zuchthygiene·S ArrighiA Pecile
Nov 21, 2016·Theriogenology·Agnieszka Leśniak-Walentyn, Anna Hrabia
May 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·M NowakA Hrabia
May 30, 2017·Domestic Animal Endocrinology·J K SochaA Hrabia
Oct 1, 2017·Theriogenology·Joanna K Socha, Anna Hrabia