PMID: 9170244Apr 1, 1997Paper

Expression of Ascaris suum malic enzyme in a mutant Escherichia coli allows production of succinic acid from glucose

Applied Biochemistry and Biotechnology
L StolsM I Donnelly

Abstract

The malic enzyme gene of Ascaris suum, was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli NZN111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. Induction of pMEA1, which encodes the native animoterminus, gave better overexpression of malic enzyme, approx 12-fold compared to uninduced cells. Under the appropriate culture conditions, expression of malic enzyme allowed the fermentative dissimilation of glucose by NZN111. The major fermentation product formed in induced cultures was succinic acid.

References

Jan 1, 1989·Journal of Bacteriology·F Mat-JanD P Clark
Dec 15, 1967·Biochemical and Biophysical Research Communications·K TakeoH Katsuki
May 21, 1971·Biochemical and Biophysical Research Communications·T MuraiH Katsuki
Sep 10, 1995·Archives of Biochemistry and Biophysics·W E BoernkeM I Donnelly
Jan 1, 1993·Archives of Biochemistry and Biophysics·G KulkarniB G Harris
May 1, 1996·Applied and Environmental Microbiology·C S MillardM I Donnelly

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Citations

Mar 7, 2006·Applied and Environmental Microbiology·Sang Jun LeeSang Yup Lee
Dec 7, 2010·Applied and Environmental Microbiology·Rintze M ZelleAntonius J A van Maris
Apr 27, 2010·PLoS Computational Biology·Sridhar RanganathanCostas D Maranas
Dec 18, 2016·Metabolic Engineering·Zachary A KingBernhard O Palsson

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