Expression of aurora kinase A correlates with the Wnt-modulator RACGAP1 in gastric cancer

Cancer Medicine
Jan BornscheinPeter Malfertheiner

Abstract

Canonical Wnt signaling is involved in gastric carcinogenesis. The aim of this study was to identify the link between Wnt signaling and aurora kinase A (AURKA), a target for the treatment of gastrointestinal cancers. Publicly available microarray data were used to identify phenotype-specific protein-protein interaction (PPI) subnetworks. The in silico analysis revealed a gastric cancer-specific PPI subnetwork consisting of 2745 proteins and 50,935 interactions. We focused on the link of AURKA to a Wnt-specific interaction module consisting of 92 proteins. There was a direct association of AURKA with Rac GTPase-activating protein 1 (RACGAP1), as well as with CTNBB1 (β-catenin) and CDKN1A as second-order interactors. Differential expression analysis revealed a significant downregulation of both AURKA and RACGAP1 in gastric cancer compared to noncancer controls. Biopsies from a prospective cohort of 56 patients with gastric cancer (32 intestinal type, 24 diffuse type) and 20 noncancer controls were used for validation of the identified targets. The RT-PCR data confirmed a strong correlation of AURKA and RACGAP1 gene expression both in the tumor, the tumor-adjacent and the tumor-distant mucosa. RACGAP1 in the tumor was also associa...Continue Reading

References

Jan 9, 2001·Virchows Archiv : an International Journal of Pathology·K MiyazawaK Mukai
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aime T FrancoRichard M Peek
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wei-meng Zhao, Guowei Fang
Feb 2, 2006·BMC Bioinformatics·Jeanette N McClintick, Howard J Edenberg
Nov 6, 2008·Cell·Victoria Sanz-MorenoChristopher J Marshall
Dec 25, 2008·Bioinformatics·Audrey KauffmannWolfgang Huber
Apr 9, 2009·Cell Biology International·Sonja RöhrsOliver Müller
Mar 17, 2010·Pediatric Blood & Cancer·Ayman El-SheikhRajeev Vibhakar
Jul 30, 2010·Digestive Diseases and Sciences·Jan BornscheinPeter Malfertheiner
Apr 12, 2011·Oncology Reports·Jing WangYuankai Shi
Aug 10, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Suk Mei WangKam M Hui
May 2, 2012·BMC Bioinformatics·Mark R DalmanZhong-Hui Duan
Apr 28, 2012·PloS One·Ignat DrozdovPeter Malfertheiner
Nov 9, 2012·Breast Cancer Research and Treatment·Karin Milde-LangoschRalph M Wirtz
Nov 30, 2012·Nucleic Acids Research·Gabriella RusticiUgis Sarkans
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Mar 26, 2013·Journal of Neuro-oncology·Hong-Lin KeXiao-Qiang Wang
Sep 11, 2013·The Journal of Cell Biology·Guillaume JacquemetPatrick T Caswell
Mar 13, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Susumu SaigusaMasato Kusunoki
May 9, 2014·Expert Opinion on Investigational Drugs·Giuseppe AprileMario Scartozzi
Jul 23, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Gowrikumar SaiprasadGanapasam Sudhandiran
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth

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Citations

Aug 31, 2018·Frontiers in Genetics·Lushun YuanXinghuan Wang
Apr 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Li YinQianfeng Xia
Nov 3, 2020·Frontiers in Molecular Biosciences·Xiaowei ChenXiaobing Shen
Jan 12, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ahmed KatshaWael El-Rifai

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Datasets Mentioned

BETA
GSE19826

Methods Mentioned

BETA
biopsies
PCR
electrophoresis

Software Mentioned

R
SPSS
STRING
GraphPad Prism
ArrayExpression
STRINGdb Bioconductor
arrayQualityMetrics
Bioconductor
limma

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