Expression of Autophagy-Related Proteins in the Spinal Cord of Pembroke Welsh Corgi Dogs With Canine Degenerative Myelopathy

Veterinary Pathology
Mizue OgawaHiroyuki Nakayama

Abstract

Canine degenerative myelopathy (DM) is a progressive neurodegenerative disease frequently found in Pembroke Welsh Corgi (PWC) dogs, and it has clinical and pathologic similarities to human amyotrophic lateral sclerosis. Autophagy is a major intracellular protein degradation system. Abnormalities of autophagy-resulting in cell death through mechanisms called type II programmed cell death-have recently been reported to occur in various neurodegenerative diseases, including amyotrophic lateral sclerosis. Thus, the distribution and expression levels of proteins involved in autophagy were examined in the spinal cords of 8 PWC dogs suffering from DM with superoxide dismutase mutation, 5 non-DM PWC dogs, and 6 Beagle dogs without neurologic signs. There was no significant difference in the ratio of neurons with microtubule-associated protein light chain 3 (LC3)-positive somata relative to those that were LC3 negative among the 3 groups, whereas the number of LC3-positive neurites was significantly increased in DM dogs. Punctate LC3 immunoreactivity did not colocalize with a lysosome marker, LAMP2 (lysosome-associated membrane protein 2). NBR1 (neighbor of BRCA gene 1) was localized mostly in reactive astrocytes, whereas there were p62...Continue Reading

References

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Citations

Oct 4, 2015·Zoology : Analysis of Complex Systems, ZACS·Raffaele NardoneEugen Trinka
Apr 26, 2016·The Journal of Veterinary Medical Science·Kanae OyakeHiroaki Kamishina
Nov 7, 2015·Veterinary Pathology·W Baumgärtner
Apr 11, 2018·Veterinary Pathology·Joshua D Webster
Nov 5, 2019·Frontiers in Genetics·Sára Sándor, Enikő Kubinyi
May 28, 2021·Veterinary Pathology·Sara Francesca SantagostinoEnrico Radaelli
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Ângela MartinsAntónio Ferreira

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Datasets Mentioned

BETA
AF346417

Methods Mentioned

BETA
electrophoresis
Protein assay
Transgenic

Software Mentioned

Elements
NIS
Image Lab

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