Expression of AV-1, a position-specific molecule, in response to retinoic acid beads and ZPA grafts in chick limb development

Developmental Biology
K Ohsugi, H Ide

Abstract

Local application of retinoic acid (RA) to the anterior-distal margin of chick limb buds causes digit pattern duplications identical to those that result from zone of polarizing activity (ZPA) grafts. The AV-1 antigen is a cell membrane glycoprotein which is expressed during chick limb bud development in a stage- and position-specific manner that correlates with cartilage pattern formation. In order to further understand the limb patterning mechanisms affected by RA and by ZPA grafts, we first examined the effects of RA treatment on AV-1 expression. We then compared the effect of RA with those of ZPA grafts. When beads soaked in varying concentrations of RA were applied to the anterior-distal margin of wing buds, changes in the pattern of AV-1 antigen expression were observed. AV-1 expression appears to correlate with regions that will give rise to a digit 3. These results suggest that the AV-1 protein is one of the molecules which may be involved in the process of cartilage pattern formation during limb development. ZPA grafts produced similar alterations in AV-1 expression, but the initial changes were observed earlier with ZPA grafts than with RA beads. These results support the idea that RA changes anterior limb bud cells i...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.