Expression of azurophil and specific granule proteins during differentiation of NB4 cells in neutrophils

Journal of Cellular Physiology
C GrégoireI Maridonneau-Parini

Abstract

Neutrophils contain several populations of secretory granules with characteristic sets of proteins. Granule proteins are sorted into their respective granule types by temporal regulation of their expression during cell differentiation and/or by specific targeting signals. We investigated the expression of some granule proteins in human promyelocytic NB4 cells. Like other myeloid cell lines which can be differentiated into neutrophils, NB4 cells lack the specific-granule population. We report here that, nevertheless, they express the specific-granule matrix protein lactoferrin, when differentiated with retinoic acid. Lactoferrin and the azurophil-granule protein beta-glucuronidase were simultaneously expressed, whereas myeloperoxidase expression had stopped, showing that azurophil-granule proteins are not all produced concomitantly. Cell fractionation by Percoll gradient revealed that while beta-glucuronidase co-fractionated with myeloperoxidase, lactoferrin was mostly contained in a vesicular compartment free of markers for azurophil granules, plasma membrane, and Golgi. This vesicular compartment was not implicated in regulated exocytosis since it was not mobilized by secretagogues, which, in parallel, induced the release of m...Continue Reading

References

Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·S RuchaudM Lanotte
Jul 15, 1993·The New England Journal of Medicine·R P WarrellL Degos
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Citations

Nov 12, 2002·Environmental Health Perspectives·Da-Tian BauKun-Yan Jan
Sep 17, 2004·Platelets·James G White, Richard D Brunning
Jan 29, 2021·Journal of Inflammation Research·Marfa BlanterSofie Struyf
Oct 3, 1999·Antimicrobial Agents and Chemotherapy·C Miossec-BartoliA Diu-Hercend
Sep 1, 2006·Leukemia Research·Malene Bjerregaard PassJack Bernard Cowland

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