Expression of bacterial L-aspartate-alpha-decarboxylase in tobacco increases beta-alanine and pantothenate levels and improves thermotolerance

Plant Molecular Biology
W M Fouad, Bala Rathinasabapathi

Abstract

L- Aspartate-alpha-decarboxylase catalyzes the decarboxylation of L -aspartate to generate Beta-alanine and carbon dioxide. This is an unusual pyruvoyl-dependent enzyme unique to prokaryotes that undergoes limited self-processing. The Escherichia coli pan D gene encoding L- aspartate-alpha-decarboxylase was expressed under a constitutive promoter in transgenic tobacco. Transgene expression was verified by assays based on RNA blots, immunoblots and enzyme activity in vitro. The pan D lines had increased levels of leaf Beta-alanine (1.2- to 4-fold), pantothenate (3.2- to 4.1-fold) and total free amino acids (up to 3.7-fold) compared to wild-type and vector controls. Growth of homozygous lines expressing E. coli L- aspartate-alpha-decarboxylase was less affected than that of the control lines when the plants were stressed for 1 week at 35 degrees C. When transferred from 35 to 30 degrees C for 3 weeks, the Pan D transgenic lines recovered significantly (P <or= 0.001) better than the controls: Pan D lines had on an average 54% and 84% greater fresh and dry weights respectively, compared to the controls. Homozygous lines expressing E. coli L- aspartate-alpha-decarboxylase had significantly greater thermotolerance (P<or=0.05) during ...Continue Reading

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Citations

Oct 13, 2007·Plant Molecular Biology·Rafal JonczykUlrich Genschel
Aug 30, 2008·Plant Molecular Biology·Ereck ChakauyaAlison G Smith
Sep 28, 2007·Natural Product Reports·Fabrice RébeilléMartin J Warren
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ching-Hui YehYee-Yung Charng
Sep 17, 2013·Physiologia Plantarum·Yu-Hsing ShenShih-Tong Jeng
Dec 25, 2007·The Journal of Biological Chemistry·Sabarinath SundaramBarry P Rosen
Jan 29, 2020·Applied Microbiology and Biotechnology·Xinyu ZouZhibin Feng
Aug 6, 2019·Frontiers in Plant Science·Anutthaman ParthasarathyAndré O Hudson
Dec 15, 2020·Acta Biochimica Et Biophysica Sinica·Mingyue FeiDongchang Sun
Oct 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdul-Hamid EmwasMariusz Jaremko

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