PMID: 8981993Jan 1, 1997Paper

Expression of bacterial mtlD in Saccharomyces cerevisiae results in mannitol synthesis and protects a glycerol-defective mutant from high-salt and oxidative stress

Journal of Bacteriology
V ChaturvediB Wong

Abstract

Polyols, or polyhydroxy alcohols, are produced by many fungi. Saccharomyces cerevisiae produces large amounts of glycerol, and several fungi that cause serious human infections produce D-arabinitol and mannitol. Glycerol functions as an intracellular osmolyte in S. cerevisiae, but the functions of D-arabinitol and mannitol in pathogenic fungi are not yet known. To investigate the functions of mannitol, we constructed a new mannitol biosynthetic pathway in S. cerevisiae. S. cerevisiae transformed with multicopy plasmids encoding the mannitol-1-phosphate dehydrogenase of Escherichia coli produced mannitol, whereas S. cerevisiae transformed with control plasmids did not. Although mannitol production had no obvious phenotypic effects in wild-type S. cerevisiae, it restored the ability of a glycerol-defective, osmosensitive osg1-1 mutant to grow in the presence of high NaCl concentrations. Moreover, osg1-1 mutants producing mannitol were more resistant to killing by oxidants produced by a cell-free H2O2-FeSO4-NaI system than were controls. These results indicate that mannitol can (i) function as an intracellular osmolyte in S. cerevisiae, (ii) substitute for glycerol as the principal intracellular osmolyte in S. cerevisiae, and (iii...Continue Reading

References

Aug 1, 1977·The Journal of Clinical Investigation·A I Tauber, B M Babior
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M C TarczynskiH J Bohnert
Jan 1, 1992·Advances in Microbial Physiology·A Blomberg, L Adler
Jan 1, 1991·Methods in Enzymology·K Köhrer, H Domdey
Nov 11, 1990·Molecular Microbiology·W JiangW G Niehaus
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Jan 1, 1984·Advances in Microbial Physiology·D H Jennings
Oct 1, 1982·The Journal of Experimental Medicine·S J Klebanoff
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Sep 24, 1982·Science·P H YanceyG N Somero
Mar 19, 1993·Science·J L BrewsterM C Gustin
May 17, 1995·Biochemical Pharmacology·B Halliwell
Oct 1, 1993·Molecular Microbiology·Willem H Mager, João C S Varela

❮ Previous
Next ❯

Citations

Jun 14, 2008·FEMS Microbiology Letters·Heriberto VélëzMargaret E Daub
Dec 24, 2013·SpringerPlus·Shekhar Chandra BishtPankaj Kumar Mishra
May 1, 2007·Functional Plant Biology : FPB·Christine M SicklerWayne Loescher
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·D B JenningsJ D Williamson
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Drauzio E N Rangel
Oct 7, 2015·Frontiers in Microbiology·Mukesh MeenaRam S Upadhyay
Nov 30, 2016·Frontiers in Cellular and Infection Microbiology·Caroline M MarcosAna M Fusco-Almeida
Oct 27, 2017·Metallomics : Integrated Biometal Science·Xulei HuangSam Fong Yau Li
Jul 31, 2018·FEMS Yeast Research·Lethiwe L MbuyaneBenoit Divol
Dec 6, 2005·Molecular Genetics and Genomics : MGG·Kentaro OhkuniAkihiko Kikuchi
Feb 25, 2010·Applied Microbiology and Biotechnology·Vicente MonederoMaría J Yebra
Nov 19, 2010·Journal of Physiology and Biochemistry·Joanna KolodziejczykBarbara Wachowicz
Aug 16, 2012·Environmental Microbiology·Zheng-Liang WangMing-Guang Feng
Apr 19, 2003·FEMS Yeast Research·Roeland CostenobleGunnar Lidén
Feb 1, 2014·The Plant Journal : for Cell and Molecular Biology·Charlotte TrontinOlivier Loudet
Feb 22, 2005·Molecular Plant-microbe Interactions : MPMI·Peter S SolomonRichard P Oliver
Jul 5, 2017·Applied Microbiology and Biotechnology·Luciana G Ruiz RodríguezFernanda Mozzi
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Jan 31, 2007·Applied and Environmental Microbiology·Victor LaderoPascal Hols
Aug 13, 2003·Eukaryotic Cell·George J G RuijterJaap Visser
Dec 11, 2003·Eukaryotic Cell·Kyoung Su Kim, Howard S Judelson
Jul 9, 2020·Biotechnology for Biofuels·Wenyang WuFilipe Branco Dos Santos
Mar 5, 2005·Applied and Environmental Microbiology·H Wouter WisselinkJeroen Hugenholtz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.