Expression of bacterial starch-binding domains in Arabidopsis increases starch granule size

Functional Plant Biology : FPB
Crispin A HowittMatthew K Morell

Abstract

Starch is a readily renewable resource that is very widely used for food and industrial purposes; however, greater variation in the functional properties of starch would further extend the use of this biodegradable polymer. Genetic engineering may provide a way to produce designer starches that have the desired properties. Starch-binding domains (SBD) from bacterial enzymes that catabolise starches have the ability to bind two helices of starch and thus have the potential to crosslink starch and / or to be used as anchors for other enzymes that can modify starch properties. In a first step towards novel modification of starch we have investigated the effect of expressing SBDs, singly and in tandem, in planta, and targeting them to the chloroplast in the model plant Arabidopsis thaliana (L.) Heynh. Transgenic plants that contained the SBD from the cyclomaltodextrin glucanotransferase (CGTase) of Thermoanaerobacterium thermosulfurigenes in the chloroplast were produced in both the wild type and the starch excess mutant (sex 1-1) backgrounds. Analysis of starch isolated from the chloroplasts of these lines revealed no significant changes in the amylose : amylopectin ratio, the chain-length distribution of debranched amylopectin or...Continue Reading

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Datasets Mentioned

BETA
BQ606794
GJ1158

Methods Mentioned

BETA
PCR
transgenic
Assay
electrophoresis
scanning electron microscopy

Software Mentioned

ChloroP

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