Expression of bacterial superantigen genes in mice induces localized mononuclear cell inflammatory responses

The Journal of Clinical Investigation
S W Dow, T A Potter

Abstract

Bacterial superantigens are potent T cell activators, and superantigen proteins have been injected into mice and other animals to study T cell responses in vivo. When superantigen proteins are injected, however, the T cell stimulatory effects cannot be confined to specific tissues. Therefore, to target superantigen expression to specific tissues, we used gene transfer techniques to express bacterial superantigen genes in mammalian cells in vitro and in tissues in vivo. Murine, human, and canine cells transfected with superantigen genes in vitro all produced superantigen proteins both intracellularly and extracellularly, as assessed by bioassay, immunocytochemistry, and antigen ELISA. Superantigens produced by transfected eukaryotic cells retained their biologic specificity for T cell receptor binding. Intramuscular injection of superantigen plasmid DNA in vivo induced an intense intramuscular mononuclear cell infiltrate, an effect that could not be reproduced by intramuscular injection of superantigen protein. Intradermal and intravenous injection of superantigen DNA induced cutaneous and intrapulmonary mononuclear cell inflammatory responses, respectively. Thus, superantigen genes can be expressed by mammalian cells in vivo. S...Continue Reading

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Citations

Jan 19, 2008·Nature Reviews. Cancer·Melissa Paoloni, Chand Khanna
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A T VellaP Marrack
Oct 24, 2007·The Veterinary Clinics of North America. Small Animal Practice·Melissa C Paoloni, Chand Khanna

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